Figure 1.
Receiver Operator Characteristic (ROC) decision plot.
ROC curve analysis obtained by using the real-time PCR Ct values (universal probe) versus urine culture results (Cut-off value of ≥105 CFU/ml).
Figure 2.
Correlation of PCR Ct values with culture results.
Based on culture, samples were categorized into two groups: UTI (≥105 CFU/ml) and no UTI (<105 CFU/ml). The universal 16S rDNA probe, of which a Ct value of 25 was set as breakpoint, was used to distinguish between positive and negative samples (line in dots). * p<0.05 (non-parametric Mann-Whitney Test).
Table 1.
Comparison of performance characteristics of the diagnostic test (real-time PCR assay, universal probe) using different PCR Ct cut-off values (25–30).
Figure 3.
Boxplot showing the correlation of PCR Ct values with bacterial load determined in culture.
Culture results were grouped into three categories: <103 CFU/ml, 103–104 CFU/ml and ≥105 CFU/ml. Statistical significance was determined performing Kruskal-Wallis Test.
Table 2.
Pathogen identification of the 122 growth-positive urine specimens.