Figure 1.
Volcano plot of the quantitative Photobacterium profundum proteomic data.
Data statistical significance (p-value plotted in a log10 scale and calculated from a one-wayANOVA, see material and methods for details) is plotted in function of the protein ratio intensity (protein intensity at 28 MPa/protein intensity at 0.1 MPa) in a log2 scale. The horizontal dashed line shows where p-value = 0.05 and the two vertical dashed lines separate proteins having an absolute fold-change of 1.5.
Figure 2.
Scatter plot of the non-transformed protein intensity plotted against their median intensity group.
In Fig. 2A, B, C, the biological triplicates grown at 0.1 MPa (replicates 1 to 3) is plotted in against the 0.1 MPa median intensity group. In Fig. 2D, E and F, the biological triplicates grown at 28 MPa (replicate 1 to 3) is plotted against the 28 MPa median group. In Fig. 2G, the median intensity at 28 MPa is plotted against the median intensity at 0.1 MPa. Slope and regression coefficients are also highlighted in each plot. The non- transformed protein intensity was extracted using Progenesis (see material and methods section for details).
Figure 3.
Protein localisation prediction based on PSORT (see material and method for detail).
The complete set of proteins has been used for localisation distribution.
Table 1.
Several proteins identified in this study and been previously reported in the literature to be piezo-sensitive.
Figure 4.
Correlation between the intensity ratio 28 MPa/0.1 MPa in log2 measured in this study at the protein level (y axis) and differentially expressed ORF 28 MPa/0.1 MPa in log2 presented in the study of Campanaro et al (2005) (ref. 4).
The open circles are associated to proteins identified in this study with a p-value >0.05 and the filled circles are associated to proteins identified in this study having a p-value <0.05. We have only genes/proteins identified in both studies being significantly differentially expressed. We have divided the graphe into 4 quadrants (I,II,III,IV) where I and III are correlated while II and IV are anticorrelated.