Table 1.
Primer names, sequences and PCR product size of selected candidate housekeeping genes.
Table 2.
Specific primers used for amplifying PCR amplicon standards.
Table 3.
The OD 260/280 ratios of extracted nucleic acid.
Figure 1.
Yields of total RNA and genomic DNA of various sample groups.
Figure 2.
The standard curves constructed for 18S (A), Act3 (B), EF1alpha (C), GAPDH (D), PUB-2 (E), RPS8 (F), TubB (G).
The results showed that amplification efficiency was between 96% and 103%, and linear correlation coefficient was >0.99.
Figure 3.
The melting curve analysis for 18S (A), Act3 (B), EF1alpha (C), GAPDH (D), PUB-2 (E), RPS8 (F), TubB (G).
Melting peaks were examined with standard samples and unkown samples (sporophytes, gametophytes and conchospores). The melting curve for each gene had only one peak.
Table 4.
Transcript numbers of candidate housekeeping genes in P. yezoensis determined by absolute quantitative analysis normalized to total RNA quantity (copies/μg).
Table 5.
Stability of candidate housekeeping gene expression in P. yezoensis (from smallest to largest difference) determined by difference across all samples.
Table 6.
Stability of candidate housekeeping gene expression in P. yezoensis (from smallest to largest difference) determined by difference between the sporophytes and the gametophytes.
Table 7.
Stability of candidate housekeeping gene expression in P. yezoensis (from smallest to largest difference) determined by difference between the sporophytes and the conchospores.
Table 8.
Stability of candidate housekeeping gene expression in P. yezoensis (from smallest to largest difference) determined by difference between the gametophytes and the conchospores.
Table 9.
Transcript numbers of candidate housekeeping genes in P. yezoensis determined by absolute quantitative analysis normalized to genomic DNA quantity (copies/μg).