Table 1.
Summary of data used for transcriptome assembly.
Figure 1.
Distribution of S. littoralis contigs annotated at GO level 2.
Figure 2.
Maximum likelihood tree of candidate odorant-binding proteins (OBPs) from S. littoralis and other Lepidoptera.
Sequences used were from B. mori [36], H. melpomene [41], H. virescens [56], [57], [58], M. sexta [37], [59] and from three other species of the genus Spodoptera (S. exigua, S. frugiperda and S. litura). Signal peptide sequences were removed from the data set. Branch support was estimated by approximate likelihood-ratio test (aLRT) (circles: >0.95) [70]. Images were created using the iTOL web server [89]. SlitOBPs are in bold and the new SlitOBPs identified in this study are in red.
Table 2.
List of S. littoralis contigs putatively involved in odorant binding.
Figure 3.
Maximum likelihood tree of candidate ORs from S. littoralis and other Lepidoptera.
Sequences used were from B. mori [3], C. pomonella [39], H. virescens [54], [55], M. sexta [37], D. plexippus [40] and H. melpomene [41]. Branch support was estimated by approximate likelihood-ratio test (aLRT) (circles: >0.95) [70]. Images were created using the iTOL web server [89]. SlitORs are in bold and the new SlitORs identified in this study are in red.
Table 3.
List of S. littoralis contigs putatively involved in chemosensory reception.
Figure 4.
Maximum likelihood tree of candidate ionotropic receptors (IRs) from S. littoralis and other insects.
Sequences used were from B. mori [11], C. pomonella [39], D. plexippus [40], D. melanogaster, Apis mellifera and Tribolium castaneum [11]. Branch support was estimated by approximate likelihood-ratio test (aLRT) (circles: >0.95) [70]. Images were created using the iTOL web server [89]. SlitIRs are in bold and the new SlitIRs identified in this study are in red.
Table 4.
RT-PCR amplifications of SlitOBP (including PBP and GOBP), CSP, GR, IR and OR transcripts in adult male and female antennae (ant) and larvae antennae, and larvae maxillary palps (palp).
Figure 5.
Distribution of chemosensory genes in S. littoralis adults and larvae.
RT-PCRs were performed on male and female adult antennae and caterpillars antennae and maxillary palps. OBP: odorant-binding protein, PBP: pheromone-binding protein, GOBP: general odorant-binding protein, CSP: chemosensory protein, OR: olfactory receptor, IR: ionotropic receptor, GR: gustatory receptor.