Figure 1.
Selected FSC histograms illustrating bimodal and monomodal distributions.
Selected FSC histograms illustrating bimodal and monomodal distributions. x-axis displays the channels used for analysis, y-axis gives the number of events for each channel. Dashed lines indicate channel 101 chosen for calculation of SphI. The median value for the respective part of the distribution is marked by short lines. For subject 11 (Full, IgG2b, d7), kurtosis = −1.21, PCD = −0.15, SphI = 2.47 (median 1 = 148, median 2 = 60). For subject 16 (Full, IgG2b, d3), kurtosis = −0.36, PCD = −0.27, SphI = 1.70 (median 1 = 129, median 2 = 76).
Figure 2.
Erythrocyte sphericity and buffer osmolarity.
Kurtosis of FSC signal of erythrocytes of 10 subjects of first study part incubated in buffer mediums with varying osmolarity. With increasing osmolarity of buffer medium, sphericity of erythrocytes decreases as indicated by decreasing kurtosis, *indicates significant difference (p<0.05) to kurtosis/sphericity values at 259 mosmol/kg and †indicates significant difference (p<0.05) to kurtosis/sphericity values at 285 mosmol/kg (Friedman-test with adjusted pairwise comparisons).
Figure 3.
Correlations between respective indicators of sphericity.
Scatterplots illustrating the relation between the respective indicators of sphericity in all samples of second study part (A,B) and isotype control samples (C,D). Relation between kurtosis and SphI appears inverse proportional.
Figure 4.
Longitudinal presentation of erythrocyte sphericity.
Kurtosis of FSC signal of IgG2b isotype control samples of CONT subjects at all time points. Values of the respective subjects are connected by dashed lines. Solid bold line connects mean values of distribution of kurtosis at the respective time point.
Table 1.
Descriptive statistics for measures of sphericity.
Table 2.
Results of correlation analysis between measures of sphericity.