Table 1.
Dechlorination of Aroclor 1260 in microcosms after 12 months of incubation.
Figure 1.
Reductive dechlorination of Aroclor 1260 in sediment-free cultures.
Congener distribution of Aroclor 1260 in the control sample (A); absolute difference in the congener distribution of Aroclor 1260 residues between the control and sediment-free culture CW-4 (B), CG-1 (C), CG-3 (D), CG-4 (E), and SG-1 (F) after 6 months incubation.
Figure 2.
Extensive dechlorination of Aroclor 1260 in sediment-free culture CG-5.
Congener distribution in the abiotic control (A) and in culture CG-5 after three months incubation (B); differences in congener distribution of Aroclor 1260 residues between the control bottles and culture CG-5 (C).
Table 2.
PCB homolog distribution in sediment-free culture CG-5 after incubating for 3 months.
Figure 3.
Putative PCB dechlorinating bacteria.
2S-DGGE characterization of Dehalococcoides/Dehalobacter populations present in PCB dechlorinating microcosms (A). Phylogenetic tree of PCB dechlorinating Dehalococcoides (closed circles), Dehalobacter (open circle), and Dehalogenimonas species (closed triangle) identified in the microcosms (B). Phylogenetic tree was calculated by neighbor-joining method using MEGA4 [46].
Figure 4.
Relative abundances of predominant bacterial genera existing in 6 sediment-free PCB dechlorinating cultures.
Note: the genera shown are having relative abundances higher than 1.0% in one or more of the sediment-free cultures. The band indicated the occurrence of the corresponding genus while the grayscale intensity indicated the relative abundance of the genus in the sample. Detailed relative abundance numbers were marked for the dehalogenating bacteria.
Figure 5.
PCB-dependent growth of Dehalococcoides/Dehalogenimonas in sediment-free cultures.
Cells were collected when observing their PCB dechlorination activities after 6 months of incubation for cultures CW-4, CG-1, CG-3, CG-4 and SG-1; and after 3 months for culture CG-5.