Figure 1.
Plant-derived BChE undergoes substrate activation by its succinylcholine substrate.
Blue line represents data fitted by nonlinear regression to fit the equation: with the following parameters (±SEM): Vmax = 2.45±0.16 µM/min, KM = 57±7 µM, Kss = 2.0±0.3 mM, and b = 2.9±0.1. kcat = Vmax/[BChE]T was calculated to be 516±33 min−1 based on the above Vmax value and [BChE] = 4.74 nM.
Figure 2.
Plant-derived BChE protects mice from SC-induced apnea.
A Respiration rate of mice treated with SC followed by administration of pBChE or saline was monitored. Symbols represent individual animal subjects (n = 3 for each group). B The Kaplan–Meier estimates of survival reflecting survival of all pBChE-treated mice as opposed to 100% mortality among control, saline-treated subjects. Comparison by the Log-rank (Mantel-Cox) test was significant (P<0.025).
Figure 3.
Plant-derived BChE facilitate recovery of guinea pigs treated with sublethal dose of SC.
Guinea pigs were injected with SC (0.167 mg/kg) followed by injection with BChE (n = 3, red) or saline (n = 3, blue) while monitoring oxygen saturation (A) and heart rate (B). Reported data points represent means±SEM. Means at each time point were compared by 2-way repeated ANOVA and P values are denoted on the graphs. Statistical analyses by t-test or 1-way ANOVA yielded very similar results. C Time to return to normal heart rate. Comparison by the Log-rank (Mantel-Cox) test was significant (P<0.025).
Figure 4.
Plant-derived BChE fully protects guinea pigs from high-dose SC-induced apnea.
Guinea pigs were injected with SC (0.334 mg/kg) followed by injection with BChE (n = 3, red) or saline (n = 3, blue) while monitoring oxygen saturation (A) and heart rate (B). Reported data points represent means±SEM. Means at each time point were compared by 2-way repeated ANOVA and P values are denoted on the graphs. Statistical analyses by t-test or 1-way ANOVA yielded very similar results. C Time to return to normal heart rate. Comparison by the Log-rank (Mantel-Cox) test was significant (P<0.025).