Figure 1.
SDS-PAGE of isolated HMW-GS fractions from three wheat cultivars (cvs.).
The subunit composition of wheat cvs. Contra (lane 2), Akteur (lane 3) and Apache (lane 4) is indicated with numbers according to the current nomenclature system [4]. Protein Mr markers are given in lane 1 (from top: myosin, β-galactosidase, bovine serum albumin, ovalbumin, carbonic anhydrase).
Figure 2.
RP-HPLC chromatogram (210 nm) of the isolated HMW-GS fraction of wheat cv. Akteur.
HMW-GS are labeled with numbers according to their current nomenclature system [4].
Table 1.
Levels of ω5-gliadins and HMW-GS in flour and isolated HMW-GS fractions of the wheat cvs. Akteur, Contra and Apache and the respective protein yields in the HMW-GS fractions.
Figure 3.
Base peak mass chromatogram from RP-HPLC-ESI-MS of the isolated HMW-GS fraction of wheat cv. Akteur.
The mobile phase contains 0.1% (v/v) TFA. HMW-GS are labeled with numbers according to the current nomenclature system [4].
Figure 4.
Mass spectra of wheat HMW-GS Dy10 (A) and Dx5 (B).
The spectra are taken from the average of scans under the peaks with retention times 9.7 min (A) and 11 min (B) from the base peak mass chromatogram after RP-HPLC-ESI-MS of the isolated HMW-GS fraction from wheat cv. Akteur. The simulated mass spectra obtained by maximum entropy deconvolution are shown as insets.
Table 2.
Relative average molecular masses (Mr) and corresponding proteins detected in the isolated HMW-GS fractions of the cvs. Akteur, Contra and Apache as determined by RP-HPLC-ESI-MS with the mobile phase containing 0.1% (v/v) TFA.
Table 3.
Comparison of the theoretical relative molecular mass (Mr) of HMW-GS calculated from their known amino acid sequences with their corresponding Mr determined by RP-HPLC-ESI-MS.
Figure 5.
Base peak chromatogram from RP-HPLC-ESI-MS of the isolated HMW-GS fraction of wheat cv. Akteur.
The mobile phase contains 0.1% (v/v) formic acid. HMW-GS are labeled with numbers according to the current nomenclature system [4].
Table 4.
Relative average molecular masses (Mr) and corresponding proteins present in the isolated HMW-GS fractions of the cv. Akteur as determined by RP-HPLC-ESI-MS with the mobile phase containing 0.1% (v/v) formic acid.
Figure 6.
Mass spectra of wheat HMW-GS Ax1 and ω5-gliadin.
The spectra are taken from the average of scans under the peak with retention time 6.2 min from the base peak mass chromatogram after RP-HPLC-ESI-MS with 0.1% (v/v) formic acid in the mobile phase. The simulated mass spectra obtained by maximum entropy deconvolution are shown as insets.