Figure 1.
Chemical structures of DNA, RNA and PNA.
Figure 2.
Chemical structures of backbone-modified PNA.
Figure 3.
Synthesis of negatively charged PNA monomer.
Figure 4.
Chemical structure of γ-substituted PNA monomers.
Table 1.
PNA, DNA and RNA Sequences.
Figure 5.
Tm vs [NaCl] for PNA:DNA 1 duplexes.
(A) PNA (nf/1neg/1pos):DNA 1. (B) PNA (nf/3neg/3pos):DNA 1. Conditions: 3 µM PNA, 3 µM DNA, 10 mM sodium phosphate buffer with added NaCl, pH 7.2. Error bars represent standard deviation of three or four independent trials.
Figure 6.
Tm vs [NaCl] for PNA:RNA 1 duplexes.
(A) PNA (nf/1neg/1pos):RNA 1. (B) PNA (nf/3neg/3pos):RNA 1. Conditions: 3 µM PNA, 3 µM RNA, 10 mM sodium phosphate buffer with added NaCl, pH 7.2. Error bars represent standard deviation of three or four independent trials.
Table 2.
Tm of PNA:DNA 1 duplexes at varying salt concentrations.*
Table 3.
Tm of PNA:RNA 1 duplexes at varying salt concentrations.*
Table 4.
Tm of PNA:DNA 1 and PNA:RNA 1 duplexes under simulated physiological buffer conditions.*
Table 5.
Thermodynamic parameters for the formation of duplexes of PNA 3neg and PNA 3pos with DNA and RNA.*