Figure 1.
Multidimensional Scaling Analysis of the bacterial profiles generated from the rumen fluid of 49 steers, progeny of ANG, CHA or HYB sires.
DNA from forty-nine steers fed low energy (LE) density diet and then switched to high energy (HE) density diet, was amplified using primers HDA1-GC and HDA2 (22 to 55% DGGE). Colours represent a particular sire breed: light blue, ANG fed LE diet; cyan blue, ANG fed HE diet; light pink, CHA fed LE diet; orange, CHA fed HE diet; azure blue, HYB fed LE diet, and purple, HYB fed HE diet. The comparison of the PCR-DGGE profiles was generated with the Bionumerics software package using UPGMA (unweighted pair-group) method as described in the text; comparison was optimised upon calculation of the best values for tolerance.
Table 1.
Taxonomic identification of breed-associated bacterial phylotypes in rumen liquid of steers feed diverging diets (n = 49).
Table 2.
Taxonomical identification of diet-associated bacterial phylotypes within particular breed cohorts (n = 49).
Table 3.
Taxonomic identification of breed-associated methanogen phylotypes in rumen liquid of steers feed diverging diets (n = 49).
Table 4.
Taxonomical identification of diet-associated methanogen phylotypes within particular breed cohorts (n = 49).
Table 5.
Phenotypic indicators of metabolic differences (RFI, DMI and FCR) and ruminal metabolic measurements in steers differing breed (n = 49) in LE.
Table 6.
Particular bacterial species and methanogen population in steers differing breed (n = 49) in LE.
Table 7.
Particular bacterial species and methanogen population in steers differing breed (n = 49) in HE.
Figure 2.
Correspondence Analysis (CA) plot displaying the interactions among frequencies of bacterial and methanogen phylotypes and sire breed.
Structural relationships among frequencies were displayed in a data cloud, where Dimension 1 indicated the frequency of either methanogen or bacterial phylotypes (column coordinates) while Dimension 2 depicts the associations of these observed frequencies and breed (row coordinates).
Figure 3.
Heat map of correlations between frequencies of bacterial and methanogen phylotypes and their relationship with diet (LE/HE) and sire breed (ANG, CHA, HYB).
Each square represents the Spearman’s correlation coefficient between the frequencies of the phylotype in the column with the frequency of the phylotype in the row. Order of phyltypes is determined as in a hierarchical cluster analysis. Self-correlations are identified in dark colour.