Table 1.
Primary antibodies used for immunohistochemistry.
Figure 1.
Immunoexpression detection (X400).
A: Immunoreactive nuclei for LPA-1 are observed in the epithelium (arrowheads) of control rat. B,C: LPA-1 immunostained dysplastic acini (B) and nondysplastic acini (C) from a cadmium-exposed rat. Many LPA-1–positive nuclei are observed (arrowheads). The number of immunoreactive nuclei observed in B and C is higher than that in A. D,E: PCNA immunoexpression in dysplastic (D) and nondysplastic acini of Cd-treated rats (E). Positive nuclei are detected (arrowheads); nevertheless, dysplastic acini show a huge number. F,G: The positive MCM7 nuclei (arrowheads) are evident in dysplastic (F) and nondysplastic acini (G). H,I: Ubiquitin expression, in H control acini has immunopositive expression in the cytoplasm (black arrowhead) and in the nucleus (white arrowheads). The nucleus interior is not uniform. Dysplastic lesions (I) present positive nucleus for ubiquitin (arrowheads). J,K: The localization of Bcl-2 immunoreactivity is similar in normal and dysplastic epithelium. Normal acini of Cd-treated rats are homogeneous in the cytoplasm (arrowheads in J). Dysplastic acinus shows mainly granular immunoreactivity in the apical border of the epithelium. L,M: DNA fragmentation test. Control acinus with abundant positive nuclei (arrowheads). Dysplastic acini show few apoptotic nuclei (arrowheads). N,O: p53 immunoreactive nuclei (black arrowheads) and cytoplasm (white arrowheads) cells are detected in the dysplastic lesions (L) and in normal epithelium (O). P,Q: Endothelial positive cells to FVIII are recognized in dysplastic stromal tissue (P) and normal acini (Q). Sections counter stained: Harris hematoxylin (A,B,C,P,Q), methyl green (D,E,H,I), and acetic carmine (F,G,J,K,L,M,N,O).
Figure 2.
Bar diagram indicating the mean ± SD of the LILPA1 in normal acini of control rats (Ctrl), normal acini of Cd-treated rats (Cd) and dysplastic acini of Cd-treated rats (Dysp Cd).
Lines and asterisks indicate statistically significant differences (**p<0.01).
Figure 3.
Bar graphs of the values of LIPCNA (A) and LIMCM7.
Results are expressed as mean ± SD. Lines and asterisks indicate statistically significant differences (*p<0.05).
Figure 4.
Mean ± SD of VFBcl-2 (A), LIAPO (B), LIp53 (C), and LIUBI (D).
Bars showing lines and asterisks on top of the error bars differ significantly (*p<0.05, **p<0.01, ***p<0.001).
Figure 5.
Bar diagram showing the mean ± SD of the length of microvessels per unit of volume (LVMV/mm3).
Statistical differences are indicated by lines and asterisks on top of the error bars (**p<0.01).
Table 2.
Pearson correlation coefficient of LPA-1 with proliferate, apoptotic, and angiogenesis markers in dysplastic acini of Cd-treated rats.