Table 1.
List of primers used in this study. Restriction sites are underlined.
Table 2.
List of strains and plasmids used in this study.
Figure 1.
Growth of S. pneumoniae D39 in the presence of 0.5% cellobiose (grey line ▪) and 0.5% glucose (black line ♦) in M17 medium.
Black circles show the time points at which cultures were harvested for transcriptome analysis. Where C = cellobiose, G = Glucose, T1 = time point 1 and T2 = Time point 2.
Table 3.
Number of genes significantly* affected in the presence of cellobiose at time point T1 and T2.
Table 4.
List of genes that are differentially expressed in the transcriptome comparison of S. pneumoniae D39 strain grown in CM17 and GM17 at time points T1 and T2.
Figure 2.
Organization of the bgu operon in S. pneumoniae D39.
Lollipop structure represents transcriptional terminator. Black arrows represent promoter regions. See text for further details.
Table 5.
Specific β-galactosidase activity (miller units) of D39 wild-type containing the PbguA-lacZ fusion grown in M17 medium with different added sugars (0.5% w/v).
Table 6.
Specific β-galactosidase activity (miller units) of D39 wild-type containing the PbguA-lacZ fusion grown in M17 medium with different combinations of added sugars (% w/v).
Table 7.
Summary of transcriptome comparison of S. pneumoniae strain D39 ΔbguR and D39 wild-type grown in GM17.
Table 8.
Specific β-Galactosidase activity (miller units) of D39 wild-type, ΔbguR, ΔccpA, and ΔbguDBC mutants all containing the PbguA-lacZ transcriptional fusion grown in M17 medium supplemented with added concentrations (0.5% w/v) of cellobiose (C) and glucose (G).
Figure 3.
Analysis of truncations of PbguA.
A schematic overview of the bguA promoter truncations is shown. The table on the right gives the specific β-galactosidase activity of the truncated promoters in GM17 (0.5% glucose+M17) and CM17 (0.5% cellobiose+M17). Standard deviation is given in parentheses. The oval indicates the position of the putative BguR operator site, while the sequence of the BguR operator site is given above.
Figure 4.
Growth of S. pneumoniae D39 wild-type (♦) and its isogenic mutants celR (▪), bguDBC (▴) and celR-bguDBC (X) in M17 (A), 0.5% glucose+M17 (B) and 0.5% cellobiose+M17 (C).