Table 1.
Summary of published marine, non-coral viral metagenomic datasets.
Figure 1.
Sampling site map for the POV dataset.
Thirty-two viral metagenomes represent discretely sampled and processed datasets that vary over time and space in the pelagic Pacific Ocean. (A) Overview of sampling sites, (B) GBR – Great Barrier Reef, Australia, near Dunk and Fitzroy Islands. (C) LineP- oceanographic transect off Vancouver Island, British Columbia (D) MBARI- Line67 oceanographic transect off of Monterey Bay, California (E) SIO- Scripps Pier, San Diego, CA. Images were created using Ocean Data View.
Table 2.
Description of POV samples and associated metadata.
Figure 2.
The POV dataset and its place in the viral protein universe.
(A) Summary superkingdom taxonomy statistics for quantitative Pacific Ocean viral metagenomes from 16 photic and 16 aphotic zone samples. Reads were taxonomically assigned based on matches to proteins in SIMAP and curated as described in the methods. (B) Venn diagram representing medium- to large membership PCs documents the relative contributions of the POV, GOS microbial, and SIMAP datasets to the ‘viral protein universe’.
Table 3.
POV ORF recruitment.
Table 4.
Distribution and count by PC size.
Figure 3.
Viral community functional richness based on season and proximity to shore.
Rarefaction analysis of hits to protein clusters from: (A) 11 POV metagenomes from a single LineP open ocean site (station P26) (B) 11 POV metagenomes from LineP stations P4, P12, and P26 from a single research cruise (June 2009). To be conservative, only protein clusters with >20 members were used in these analyses.
Figure 4.
Viral community functional richness in the Pacific Ocean.
Rarefaction analysis of hits to protein clusters from all POV metagenomes in (A) photic zone samples and (B) aphotic zone samples. To be conservative, only protein clusters with >20 members were used in these analyses.
Table 5.
Percentage of POV reads assigned to superkingdoms.