Figure 1.
Map of China and the Bohai Sea Ring areas,Shandong Province.
The Laizhou Bay, Shandong Province (B:areas enclosed by the black circle), where the 2 human isolates (LZ-HGA-agent-3 and LZ-HGA-agent-4) and the tick isolate (LZ- HGA-agent-T1) isolated. The H. longicornis collected in the Laizhou Bay (D and E). Images of HGA patients with eschars from the Laizhou Bay, Shandong Province (F and G).
Table 1.
Sequences used in phylogenetic analysis based on the 16S rRNA gene and ank A gene of A. Phagocytophilum.
Figure 2.
Morules in the neutrophils of HGA patients and morulae in the cultured HL60 cells.
Morules(Wright's stain, mag×1000) in the neutrophils of the Patient15(A) and the Patient 37(B); Morulae (Wright's stain, mag×1000) in the cultured HL60 cells of the Patient 15(C) and the Patient 37(D) and morules(IFA,mag×600). in the cultured HL60 cells of the Patient 15(E) and the Patient 37(F).
Table 2.
Clinical and laboratory findings and laboratory diagnosis data in 46 confirmed and 16 probable cases in China, 2009–2010.
Table 3.
Compare of Laboratory markers between the patients in Shandong Laizhou Bay and the patients in other 3 areas in the study.
Figure 3.
Phylogenetic tree based on the partial sequences of A. phagocytophilum 16S rRNA gene.
Blue sequences: the five Chinese isolates of A. phagocytophilum identified in this study. Red sequences: H. longicornis isolates collected from the patients' domestic animals in Laizhou Bay, Shandong Province. Black sequences: sequences from patients, domestic animals, wild animals and ticks from other parts of the world. Green sequences:sequences from patient in Anhui Province in 2006 and patient in Yiyuan County, Shandong Province in 2007.
Figure 4.
Phylogenetic tree based on the partial sequences of A. phagocytophilum ank A gene.
Blue sequences:BJ-HGA-agent-1, CZ-HGA-agent-2, LZ-HGA-agent-3, LZ-HGA-agent-4, and LZ- HGA-agent-T1) and some A. phagocytophilum isolates identified in patients, domestic animals, wild animals and ticks from other parts of the world.