Figure 1.
Effect of α-santalol on cell viability and proliferation.
Human breast cancer cells MCF-7 and MDA-MB-231 and normal human breast epithelial cells MCF-10A were treated with either DMSO (control) or 10–100 µM α-santalol for 12, 24 and 48 h. At the end of respective treatments, MTT and BrdU incorporation assays were performed on each cell line. Data in left panels (A–C) were obtained from MTT assays and data in right panels (D–F) were from BrdU cell proliferation ELISA of MCF-7, MDA-MB-231 and MCF-10A cells respectively. Values were shown as mean ± SD of at least three experiments. *, P<0.05 indicates statistically significant decrease in α-santalol treated groups as compared with the control.
Figure 2.
Effect of α-santalol on DNA fragmentation by TUNEL assay and flow cytometry.
(A) MCF-7, (B) MDA-MB-231 and (C) MCF-10A cells were treated with α-santalol (0–100 µM) for 48 h and the extent of DNA fragmentation was determined by flow cytometric analysis. APO-BrdU TUNEL assay kit (Invitrogen) was used for the experiment and BrdU incorporation at DNA strand breaks of apoptotic cells were detected by conjugation to an Alexa Fluor 488 dye-labeled anti-BrdU antibody. The extent of DNA fragmentation was quantified by computational analysis of cells staining positive for BrdU using CellQuest software. (D) The bar graph indicates the percentages of apoptotic cells with fragmented DNA in MCF-7, MDA-MB-231 and MCF-10A cells. In each case data represents mean ± SD of three observations. *, P<0.05 indicates statistical significance in α-santalol treated groups compared with the control.
Figure 3.
Effect of α-santalol on cell cycle progression in MCF-7 cells.
Cells were treated with DMSO (control) or 25–75 µM of α-sanatlol for 12 and 24 h, stained with propidium iodide and distribution of cells in different phases of cell cycle were analyzed by flow cytometer. Histograms representing the fluorescence pattern for cell cycle distribution in different treatments are shown for 12 h (A) and 24 h (B). The percentage of cells in each cell cycle phases after 12 h and 24 h respectively are shown in (C) and (D). Data shown here are representative of those having similar results.
Figure 4.
Effect of α-santalol on cell cycle progression in MDA-MB-231 cells.
Data were obtained as explained in figure 3. Representative histograms of the fluorescence pattern for cell cycle distribution in different treatments after 12 h (A) and 24 h (B) are shown. (C) and (D) are the percentages of MDA-MB-231 cells in each cell cycle phases after 12 and 24 h α-santalol treatment.
Figure 5.
α-Santalol induces activation of different caspases and PARP cleavage in MCF-7 cells.
Total cell lysates were prepared from the cells treated with the indicated concentration of α-sanatlol for 12 and 24 h. Equal amounts of proteins were separated by SDS-PAGE and subjected to western immunoblotting. Membranes were probed with respective primary antibodies followed by appropriate secondary antibody and protein expression was determined by ECL detection system. β-actin was used to verify equal loading of the samples.
Figure 6.
α-Santalol induces apoptosis in MDA-MB-231 cells by activating different caspases and PARP cleavage.
Total cell lysates were made and the expressions of various apoptotic proteins were determined by western immunoblotting. β-actin was used as an internal control.
Figure 7.
Western Blot analysis of the cell cycle regulatory proteins.
(A) MCF-7 and (B) MDA-MB-231 cells were treated with α-santalol (0–75 µM) for 12 and 24 h, and equal amounts of proteins were subjected to immunoblot for the detection of the indicated G2/M regulatory proteins. (C) and (D) show the effect of α-santalol on p21 and p53 expression in MCF-7 and MDA-MB-231 cells respectively. Blots were probed for β-actin to ensure equal protein loading.