Figure 1.
Membrane Conductance of α-hemolysin.
Representative impedance spectroscopy recordings of 50 nM α-hemolysin added to tethered membranes made from AM199 lipid containing varying amounts of cholesterol (mol% chol) (A) 0, (B) 10, (C) 20, (D) 30, (E) 40, (F) 50; [n = 3].
Figure 2.
Membrane Conductance of Lysteriolsin.
Representative impedance spectroscopy recordings of (A-E) 2 µM Lysteriolysin and (F) buffer control, added to tethered membranes made from AM199 containing varying amounts of cholesterol (mol% chol) (A) 50, (B) 25, (C) 12.5, (D) 6.25, (E) 0, (F) 50; [n = 3].
Figure 3.
Membrane Conductance of CLIC1.
A. Representative impedance spectroscopy recordings of (A-E) 7.4 µM CLIC1 and (F) buffer control, added to tethered membranes made from AM199 lipid containing varying amounts of cholesterol (mol% chol) (A) 50, (B) 25, (C) 12.5, (D) 6.25, (E) 0, (F) 50; [n = 3]. B. Representative impedance spectroscopy recordings of (A) buffer control, (B) 7.4 µM boiled CLIC1 and (C) 14.8 µM boiled CLIC1, added to tethered membranes made from AM199-PC lipid containing 25 mol% cholesterol; [n = 3].
Figure 4.
Effects of Cholesterol on the Membrane Conductance of CLIC1.
Representative impedance spectroscopy recordings of (A) 7.4 µM CLIC1 (in 100 µL volume) pre-incubated with 2 µL ethanol (solvent control), (B) no protein added (buffer control), (C) 7.4 µM CLIC1 (in 100 µL volume) pre-incubated with 2 µL cholesterol in EtOH, and (D) 14.8 µM CLIC1 (in 100 µL volume) pre-incubated with 2 µL cholesterol in EtOH. Samples added to tethered membranes made from AM199 lipid containing 50 mol% cholesterol; [n = 3].
Figure 5.
Insertion of CLIC1 into Lipid Monolayers.
Representative area traces of Langmuir films held at a surface pressure of 20 mN/m with 0.036 µM CLIC1 (A-C) and (D, E) no CLIC, added beneath POPC monolayers containing cholesterol (mol% chol) (A) 16.7, (B) 9.0, (C) 0.0, (D) 16.7, (E) 0.