Figure 1.
Germination process of soybean and rice seeds.
A) water absorption curve during soybean seed germination; B) images of rice and soybean seeds during germination. Bars are 1 mm in rice images and 4 mm in soybean images.
Figure 2.
SDS-PAGE gel image of the germinating soybean seeds.
12% gel was used, and 4 ug of marker proteins were loaded. The s1–s10 indicate the 10 slices that were cut for in-gel digestion.
Figure 3.
Functional categorization of the identified proteins.
(A) total proteins; (B) the metabolism related proteins.
Table 1.
Sub-categorization of the metabolism related proteins in germinating seeds of soybean and rice.
Figure 4.
Dynamic changes of fatty acids during germination of soybean and rice seeds.
(A) total fatty acids; (B) different compositions of fatty acids. Values were means of triplicate±SD.
Figure 5.
Dynamic changes of the free amino acids during germination of soybean and rice seeds.
A) total free amino acids; B) different individual amino acids. Values were means of triplicate±SD.
Figure 6.
TEM images of soybean seeds showing the accumulation of starches at the late stage of soybean seed germination.
(A) the dry seeds; (B) 3-day-imbibition seeds. PSV, protein stocking vesicle; OB, oil body; S, starch granule; V, vacuole. Bars' lengths are 5 um.
Figure 7.
Accumulation of ROS in germinating crop seeds.
(A) soybean; (B) rice. The upper panel shows the accumulation of H2O2 with TMB staining, the bottom panel shows the accumulation of superoxide anions.