Figure 1.
The introduced Bluestripe Snapper Lutjanus kasmira and the parasitic nematode Spirocamallanus istiblenni.
Photo credits: Greta Aeby, Keoki Stender, and Chelsea Wood.
Figure 2.
Map of sample locations for three species of host fish.
Abbreviations for sample locations, species, and number of specimens collected are listed in Table 1. Colored circles delineate the two divergent lineages detected in phylogenetic analyses (Fig. 3). Pie charts for Hawai'i locations represent prevalence data for the host fish Lutjanus kasmira (black = proportion infected; white = proportion not infected). Main Hawaiian Islands listed include HI, MA, OA, and KA.
Table 1.
Summary statistics for spirocamallanids collected from three host species.
Table 2.
Molecular diversity indices for COI sequences from Spirocamallanus istiblenni.
Figure 3.
Phylogenetic trees for 18S and ATPSβ alleles and COI haplotypes from Spirocamallanus istiblenni.
The best maximum likelihood tree generated using program default settings in mega 5 [31]. Bootstrap support values were calculated using default settings with 1000 replicates. For comparison Bayesian posterior probabilities are presented. Colored bars delineate the two divergent lineages detected in French Polynesia and Hawai'i (green) in the Northern Line Islands (brown).
Figure 4.
Median Joining network for 383 COI sequences of Spirocamallanus istiblenni.
Only specimens collected from French Polynesian (Marquesas and Society Islands) and Hawai'i are shown. Each circle represents one mitochondrial haplotype with the area of each circle proportional to number of that particular haplotype in the data set; colors represent sampling location (see key).
Table 3.
Haplotype frequencies for the cytochrome oxidase I gene (COI) for Spirocamallanus istiblenni.
Figure 5.
Rarefaction curves plotting the number of individuals sampled against the expected number of mitochondrial haplotypes.
Samples from the source populations at the Marquesas (a) and Society (b) Islands are plotted separately. Grey lines represent data from source populations, black lines represent data from Hawai'i, and dashed lines are 95% confidence intervals.