Figure 1.
CP-D demonstrates a significantly elevated ECAR than other BE cell lines.
Shown above are mean measures of total (a) ECAR/cell and (b) OCR/cell. Error bars represent standard-deviation of mean between experiments (N = 6–11). Each experiment consisted of 3 to 4 replicate wells per cell line with four serial measures performed on each well. p-values (Tukey-Kramer test) of statistically significant differences from CP-A are shown.
Figure 2.
CP-D displays a greater OCR and ECAR response to glycolytic inhibition than other cell lines.
Following addition of 50 mM 2-DG, total (a) ECAR and (b) OCR were measured on the Seahorse XF24 analyzer for each cell line and changes versus untreated baseline are plotted. Error bars represent standard-deviation of means between experiments (N = 2–4). Each experiment consisted of 3–4 replicate wells per cell line with four serial measures performed on each well. p-values (Tukey-Kramer test) of statistically significant differences from CP-A are shown.
Figure 3.
CP-C and CP-D display a lower ECAR increase after mitochondrial uncoupling than other cell lines.
Following addition of 50 µM 2,4-DNP, total (a) ECAR and (b) OCR were measured on the Seahorse XF24 analyzer for each cell line and changes versus untreated baseline are plotted. Error bars represent standard-deviation of means between experiments (N = 2–4). Each experiment consisted of 3–4 replicate wells per cell line with four serial measures performed on each well. p-values (Tukey-Kramer test) of statistically significant differences from CP-A are shown.
Figure 4.
CP-C and CP-D demonstrate a stronger Crabtree effect response than other cell lines.
Following the increase of glucose concentration in media from 0 mM to 5 mM, total (a) ECAR and (b) OCR were measured on the Seahorse XF24 analyzer for each cell line and changes versus untreated baseline are plotted. Error bars represent standard-deviation of means between experiments (N = 2–4). Each experiment consisted of 3–4 replicate wells per cell line with four serial measures performed on each well. p-values (Tukey-Kramer test) indicating significant differences from CP-A are shown.
Figure 5.
Barrett's esophagus progression to esophageal adenocarcinoma involves an intermediate metabolic stage with increased glycolysis and functional mitochondria.
Early-stage BE cells (e.g. CP-A), rely mainly on mitochondrial oxidative phosphorylation for energy needs prior to the glycolytic increase which occurs in late-stage BE cells (CP-B, CP-C and CP-D), which demonstrate elevated ECAR (all) and the Crabtree effect (CP-C and CP-D). Finally in esophageal adenocarcinoma (OE-33) mitochondrial uncoupling occurs with increased OCR and glycolysis.