Figure 1.
The structure and molecular weight of resveratrol (A) and FUDR (B).
Figure 2.
The summary of drug administration for LB medium method, NGM live method, NGM dead method, and spot dead method.
Figure 3.
The HPLC profile and retention time of resveratrol and FUDR extracted from the day 1 worms.
The retention time of resveratrol (A) and FUDR (B) in the worms was 7.2 min and 5.2 min, respectively.
Table 1.
The repeatability and stability of the HPLC method.
Table 2.
The precision of the HPLC detection of resveratrol and FUDR.
Figure 4.
The drug absorption efficiency of worms administrated with 100 µM resveratrol (A) or 50 µM FUDR (B) by five delivering methods (µg/g).
The concentration of drugs in worms was presented as µg/g. The figure showed the average of three repeated experiments for each method. The details of data were summarized in Table S1 and S2.
Table 3.
The absorption efficiency of worms compared between the NGM dead method and the liquid growing method.
Figure 5.
The resveratrol (A) and FUDR (B) catabolism rate inside the worms within 16 hours (µg/g).
The worms were cultured by using NGM dead method for 6 hours, then transferred to NGM plates containing no resveratrol or FUDR. The worms were harvested at the 10 min, 30 min, 1 hr, 2 hr, 3 hr, 4 hr, 6 hr, 8 hr, 12 hr and 16 hr after transferring respectively. The details of data were summarized in Tables S3 and S4.
Figure 6.
Resveratrol (A) and FUDR (B) concentration in the medium of NGM dead method, NGM live method and LB medium method within 12 hours (mg/L).
The initial concentration of resveratrol or FUDR in the medium was 100 µM or 50 µM (0 hr). The medium was crushed and transferred into 15 mL tube at the 0.5 hr, 1 hr, 3 hr, 6 hr and 12 hr after preparing respectively. The same volume of methanol was added into the tube. The mixture was sonicated for 1 hour, then the liquid was collected for HPLC analysis. The details of data were summarized in Table S5.