Figure 1.
BALf, lung, heart, brain and plasma immunoblotings.
Western blottings displaying proteins in BALf (A), lung (B), heart (C), brain (D) and plasma (E) in sham and PM10sum-treated mice, 24 h after the third intratracheal instillation. Reported blots are representative of 6 sham and 6 PM10sum-treated mice; densitometry analyses are reported in Tab.1.
Table 1.
BALf analysis.
Table 2.
Lung gene expression.
Table 3.
Immunoblotting analysis.
Table 4.
Blood gene expression.
Table 5.
Blood/plasma analysis.
Figure 2.
Histology of lung tissue 24 h after the third intratracheal instillation. (A, B): lung parenchyma of sham mice, showing bronchiolar and alveolar epithelia; (C, D): PM10sum-treated lung parenchyma, showing inflammatory cells recruitment and AMs infiltration (arrows) in the connective surrounding terminal bronchioles and proximal alveolar sacs. Each figure represents the status evidenced examining 6 sham and 6 PM10sum-treated mice. A and C bars = 150 µm; B and D bars = 50 µm.
Figure 3.
Lung immunohistochemical analysis.
Immunohistochemical analyses of HO-1 expression in lung tissue 24 h after the third intratracheal instillation; the brown color reflects the site and intensity of HO-1 expression. (A): lung of sham mice; in the box, a control of the immunohistochemical reaction specificity, where the primary antibody has been avoided, in a PM10sum instilled mouse; (B): alveolar and peribronchiolar spaces in the lung of a sham mouse; (C, D): alveolar and peribronchiolar spaces in lungs of PM10sum-treated mice. Each figure represents the status evidenced examining 6 sham and 6 PM10sum-treated mice. A bar = 150 µm; B–D bars = 50 µm.