Figure 1.
Fiber morphology of contemporary bark-cloth samples from different Polynesian islands.
Bark-cloth samples were viewed directly under 10×magnifications. A.-Bark-cloth from Easter Island, Sample 1, B.- Bark-cloth from Easter Island, Sample 2, C.- Bark-cloth from the Marquesas archipelago, D.- Bark-cloth from Samoa, E.- Bark-cloth from Tonga, F.- Bark-cloth from Hawaii.
Figure 2.
Comparison of DNA yield extracted from fresh tissues from an individual B. papyrifera plant and from bark-cloth from Easter Island.
Yields obtained from DNA extractions of leaves, root and fresh bark. 1 H: One hour treatment with cellulase; 3 H: 3 hours treatment with cellulase; EI1: Easter Island sample 1; EI2: Easter Island sample 2. Standard deviations from triplicates are indicated.
Figure 3.
Absorption profiles of DNA extracted from fresh B. papyrifera tissues and bark-cloth samples.
A: Negative control; B: B. papyrifera (leaf); C: B. papyrifera (bark); D – I: B. papyrifera bark-cloth from D: Easter Island, sample 1; E: Easter Island, sample 2; F: Marquesas; G: Samoa; H: Hawaii; I: Tonga.
Table 1.
Details of the bark-cloth samples used in the study.
Figure 4.
Amplification of the ITS region using DNA obtained from bark-cloth.
A.- Complete ITS region, lane 1∶100 bp DNA Ladder, lane 2: Morus (leaf), lane 3: B. papyrifera 1 (leaf), lane 4: B. papyrifera 2 (leaf), lanes 5 to 9: bark-cloth samples. Lane 5: Easter Island 1, lane 6: Easter Island 2, lane 7: Tonga, lane 8: Marquesas, lane 9: Samoa, lane 10: H2O. B.- ITS1 region, lane 1∶100 bp DNA Ladder, lane 2: Morus (leaf), lane 3: B. papyrifera 1 (leaf), lane 4: B. papyrifera 2 (leaf), lanes 5 to 9: bark-cloth samples. Lane 5: Easter Island 1, lane 6: Easter Island 2, lane 7: Tonga, lane 8: Marquesas, lane 9: Samoa, lane 10: Negative control, lane 11: H2O. C.- ITS1 region, lane 1∶100 bp DNA Ladder, lane 2: Morus (leaf), lane 3: bark-cloth from Hawaii, lane 4: Negative control, lane 5: H2O.
Figure 5.
Amplification of the microsatellite SS05 locus using DNA obtained from different B. papyrifera tissues and bark-cloth.
Lane 1∶100 bp DNA Ladder, lane 2: Morus (leaf), lane 3: B. papyrifera (leaf), lane 4: B. papyrifera (fresh bark), lane 5: B. papyrifera (root), lane 6: B. papyrifera (root hair), lane 7: Easter Island bark-cloth sample 2, lane 8: Negative control, lane 9: H2O.