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Figure 1.

Study selection procedure for peer-reviewed literature from 1995–2012.

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Table 1.

Summary of published studies by income group, as per World Bank classification [5][30].

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Table 2.

Quality assessment of the studies (1 = yes; 0 = no).

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Figure 2.

Unit cost in 2010 USD for smear test alone.

(1) Cost data were sorted by WHO regions: African Region (AFR), Region of the Americas (AMR), Eastern Mediterranean Region (EMR), European Region (EUR), South-East Asia Region (SEAR) and Western Pacific Region (WPR). (2) For studies with available information on test methods, we labeled them at the end of each bar. (3) [] indicates publication year when data collection year is not available. (4) ZN: Ziehl-Neelsen; FM: fluorescence microscopy; LED: light-emitting diode. a$1.16 is the average laboratory costs on 1000 subjects and three specimens. b$1.57 is the average laboratory costs on 1000 subjects and three specimens. c$1.88 is the total cost $26.27 divided by 14 sputum smears. dSum of the overhead cost ($10.4) and the material cost ($0.1). eFor the examination of three sputum specimens, the cost per patient evaluated is $3.24 for FM and $3.59 for ZN. fThe unit cost is the average over six regional estimates. For detailed information of the six regional estimates, see Table S2.

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Figure 3.

Unit cost in 2010 USD for culture test alone.

(1) Cost data were sorted by WHO regions: African Region (AFR), Region of the Americas (AMR), Eastern Mediterranean Region (EMR), European Region (EUR), South-East Asia Region (SEAR) and Western Pacific Region (WPR). (2) For studies with available details on test methods, we labeled them at the end of each bar. (3) “[]” indicates publication year when data collection year is not available. (4) LJ: Löwenstein-Jensen; MGIT: Mycobacteria Growth Indicator Tube; HLJ: Homemade Löwenstein-Jensen; CLJ: Commercially Löwenstein-Jensen; MMGIT: Manually Mycobacteria Growth Indicator Tube; AMGIT: Automated Mycobacteria Growth Indicator Tube; FIND: Foundation of innovative New Diagnostics; BD: Becton Dickinson. a$7.08 is the average costs between negative and positive tests. bThe paper indicates cost for organism identification per positive culture on MGIT was $37.55 for using standard biochemical tests, $16.18 for anti-MPB64 assay and $2.38 for cording; we added each of them to the cost per MGIT ($17.37) for calculating the cost for positive culture. c$9.25 is the total cost of $85.07 divided by 9.2 sputum cultures. dSum of the cost for sputum collection ($19.12) and the cost for bacterial culture ($19.99). eSum of the overhead cost ($10.4) and the material cost ($27.33). fThe unit cost is the average over six regional estimates. For detailed information of the six regional estimates, see Table S2.

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Figure 4.

Unit cost in 2010 USD for combined smear and culture test.

(1) Directly obtained cost data are in red; imputed cost data are in blue. (2) Cost data were sorted by WHO regions: African Region (AFR), Region of the Americas (AMR), Eastern Mediterranean Region (EMR), European Region (EUR), South-East Asia Region (SEAR) and Western Pacific Region (WPR). (3) For studies with available details on test methods, we labeled them at the end of each bar. (4) [] indicates publication year when data collection year is not available. (5) AFB: acid-fast bacillus; LJ: Löwenstein-Jensen; MGIT: Mycobacteria Growth Indicator Tube. aLaboratory running cost is $14.34. Estimated costs incurred by patients are $12.47 (assuming that for taking an examination, a patient has to miss one-day work, take two-way transportation and have one meal outside). b$31.55 is the total cost of $94.66 divided by three combined smear and culture tests.

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Figure 5.

Summary of estimates of the three types of tests.

(1) In each boxplot, dots represent outliers which are beyond the interval of (Q1–1.5*IQR, Q3+1.5*IQR): Q1 is the 25th percentile, Q3 is the 75th percentile, IQR is the interquartile range (75%–25%). (2) The five values listed beside each boxplot represent upper adjacent value (maximum value after excluding outliers), 75th percentile, median (50%), 25th percentile, and lower adjacent value (minimum value after excluding outliers), respectively. For instance, in Plot 5a, for estimates of smear test alone, $3.54 (upper adjacent value) is the maximum value excluding three outliers. $2.54 is the value at the 75th percentile. $1.67 is the value of median. $1.21 is the value at the 25th percentile. $0.26 (lower adjacent value) is the minimum value excluding outliers. (3) For Plot 5c, the estimates of combined test include the imputed values.

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Table 3.

Ratio of unit cost for culture to smear.

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Table 4.

Tradable cost and non-tradable cost from eight studies.

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