Table 1.
List of the proteins putatively localized in the mitochondrion-like organelle of Trimastix pyriformis.
Figure 1.
Over-expression of Trimastix proteins in yeast.
The over-expression of GFP tagged proteins of Trimastix in Saccharomyces cerevisiae. The columns represent the signals from GFP tag (green), MitoTracker (red), merged GFP and MitoTracker and DIC. Rows represent individual proteins: cpn60, P1-protein of GCS, H-protein of GCS and H-protein of GCS truncated of the first 16 amino acids.
Figure 2.
H-protein of GCS localizes into vesicles (putative mitochondrion-like organelles) in Trimastix pyriformis.
A) Immunofluorescence microscopy of the Trimastix pyriformis cell. The green signal from antiH-protein (human) co-localizes with red signal from the antiH-protein (Trimastix). The DNA is stained blue with Hoechst. B) Western blot on the cellular fractions of Trimastix pyriformis. The lines represent pure bacteria Citrobacter sp. from the culture (Bact), high speed pellet of Trimastix (HSP), supernatant of Trimastix (Sup), total lysate of Trimastix (Total).
Figure 3.
Schematic representation of protein import machinery in Trimastix pyriformis mitochondrion-like organelle.