Table 1.
Properties of fluorescent dyes.
Figure 1.
In situ detection of cellular functions in cyanobacterium Phormidium
autumnale. a–l. Cultures of Phormidium autumnale 845 CCALA grown on BG-11 medium. a. SYTOX Green staining of formaldehyde-pretreated filaments; b. variable staining of live samples after 30 min incubation with 5 µM SYTOX Green; c–d. fluorescence and bright field image of CTC-stained filaments in active phase of growth; e. partially de-activated cells stained with CTC; f–g. INT-treated samples post-stained with SYTOX Green, cells that accumulated INT-formazan (f, arrowheads) were also SYTOX-positive (g, arrowheads); h. INT-stained filaments in logarithmic phase of growth; i. disintegration of filaments after 24-h incubation with CTC; j. pattern of CTC-formazan deposition; k. DAPI-stained nucleoids in living filaments; l. yellow-green metachromatic inclusions in DAPI-stained filaments viewed with a long pass emission filter; m. reduced fluorescence intensity of DAPI-stained nucleoids in cells accumulated CTC-formazan (natural samples); n. cells from old laboratory cultures simultaneously stained with DAPI, CTC and SYTOX Green under UV-illumination, extensively damaged cells lack SYTOX Green and DAPI staining of nucleoids (arrowheads) or have whole-cell DAPI signal (asterisk); o–t. Field-collected samples of mats in July and September; o. accumulation of INT-formazan by the terminal cells of filaments; p. a filament with thick sheath stained simultaneously with CTC (q), SYTOX Green (r), and DAPI (s), and showing pigment autofluorescence (t). Scale bars are 20 µm.
Figure 2.
Living 3-month-old Chroococcidiopsis 041 CCALA laboratory culture (a) simultaneously stained with SYTOX Green (b), CTC (c) and DAPI (d) dyes, and showing pigment autofluorescence (e).
Table 2.
Classification of cells according to the selected criteria.