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Figure 1.

Clinical cases and deaths of Japanese encephalitis in Republic of Korea, 1984–2010.

Figure is based on data provided by the Korea Center for Disease Control and Prevention, 2011.

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Figure 2.

Collection sites and of Japanese encephalitis virus-positive pools, Republic of Korea, 2010.

Abbreviations in parentheses indicate the number of Japanese encephalitis virus-positive pools by mosquito species.

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Table 1.

Strains of Japanese encephalitis virus reported on GenBank and used in phylogenetic analysis of pre-membrane protein coding genes.

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Table 2.

Strains of Japanese encephalitis virus reported on GenBank and used in phylogenetic analysis of envelope protein coding genes.

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Table 3.

Total number of culicine mosquitoes collected at 6 localities in four provinces in Republic of Korea in 2010 and number of Japanese encephalitis virus positive pools (up to 30 mosquitoes) as detected using RT-PCR.

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Figure 3.

Phylogenetic tree illustrating the genetic relationship of nucleotide sequences for pre-membrane protein genes of Japanese encephalitis virus (JEV) strains identified in mosquitoes, Republic of Korea, 2010 (indicated in bold font) and reference sequences from other geographic regions as reported on GenBank.

Genotypes of JEV strains are indicated on the right of the phylogenetic tree and were assigned according to Chen et al. [45], [46]. Bootstrap support values are shown. The scale bar indicates the number of mutations. Abbreviations for strains reported in this study are as follows: K10CT = Republic of Korea (ROK), 2010, Culex tritaeniorhynchus; K10CB = ROK, 2010, Culex bitaeniorhynchus; and K10CP = ROK, 2010, Culex pipiens. Vaccine strains that have been used in ROK are indicated in bold font and with an asterisk (*).

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Figure 3 Expand

Figure 4.

Phylogenetic tree illustrating the genetic relationship of nucleotide sequences for envelope protein coding genes of Japanese encephalitis virus (JEV) strains identified in mosquitoes, Republic of Korea, 2010 (indicated in bold font) and reference sequences for JEV strains from other geographic regions as reported on GenBank.

Genotypes of JEV strains are indicated on the right of the phylogenetic tree and were assigned according to Chen et al. [45], [46]. Bootstrap support values are shown. The scale bar indicates the number of mutations. Abbreviations for strains reported in this study are as follows: K10CT = Republic of Korea (ROK), 2010, Culex tritaeniorhynchus; K10CB = ROK, 2010, Culex bitaeniorhynchus; and K10CP = ROK, 2010, Culex pipiens. Vaccine strains that have been used in ROK are indicated in bold font and with an asterisk (*).

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Figure 4 Expand

Table 4.

Comparison of amino acids differences in the envelope protein between the Japanese encephalitis vaccine strains that have been used in Republic of Korea and those identified in mosquitoes for this study.

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Table 4 Expand