Figure 1.
Geographical distribution of sampling sites in the hot waters of the Basse-Terre of Guadeloupe.
(1) the Bains Jaunes on the volcano near Saint Claude, (2) the Bains des Amours, (3) Capes River, (4) the bath of Dolé and Dolé Amont and (5) Dolé Escalier in Gourbeyre.
Table 1.
Survey of free-living amoeba isolated from geothermal recreational waters of Guadeloupe on a montly basis (2011).
Table 2.
Survey of free-living amoeba isolated from geothermal recreational waters of Guadeloupe on a montly basis (2012).
Figure 2.
Levels of Free_living amoebae according to the type of water in Guadeloupe in 2011–2012.
(A) free-living amoebae and (B) and Naegleria fowleri.
Figure 3.
Levels of Free-living amoebae according to the temperature of water in Guadeloupe in 2011–2012.
(A) free-living amoebae and (B) Naegleria fowleri.
Table 3.
Number of Naegleria fowleri isolated from the most frequented baths of Guadeloupe according to temperature and pH (2011–12).
Table 4.
Number of Naegleria fowleri isolated from the most frequented baths of Guadeloupe according to physicochemical characteristics (2011–12).
Figure 4.
PCR results for the three major thermophilic amoeba species found in Guadeloupe.
PCR detection and agarose gel electrophoresis of ITS rDNA of N. fowleri (A), N. lovaniensis (B) and Hartmannella sp. (C). M : 100 bp ladder, PC : Positive control (DNA of Naegleria fowleri (#. 359)) gives a single PCR product of 359 bp with the NFITS primers and a 448 bp with the ITS primer set. N.f : Naegleria fowleri, N.l : Naegleria lovaniensis, the ITS amplification gives a 403 bp PCR product while no band was obtained with the NFITS primers, Hart. : Hartmannella sp., the ITS amplification gives a 900 bp while no band was observed with the NFITS primer set.
Table 5.
ITS1 sequences of the three isolates identified in the different geothermal waters of Guadeloupe.
Table 6.
Distribution of sequenced isolates from the different geothermal baths of Guadeloupe.