Figure 1.
Length distribution of Tenebrio molitor unigenes.
Horizontal axis represents the length of unigenes and vertical axis represents number of unigenes.
Table 1.
Sequence statistics of the Illumina sequencing assembly.
Figure 2.
Homology analysis of Tenebrio molitor unigenes.
(A) E-value distribution. (B) Species distribution. All unigenes that had BLASTX annotations within the NCBI nr database with a cut-off E-value of 10−5 were analyzed. We used the first hit of each sequence for analysis.
Figure 3.
Gene ontology (GO) classification of Tenebrio molitor unigenes.
Categories for (A) biological processes, (B) cellular components, and (C) molecular processes. The data presented represent the level 2 analysis. The percentage of a specific category of genes in that main category is shown.
Figure 4.
Clusters of orthologous groups (COG) classification of Tenebrio molitor unigenes.
A total of 13,599 produced functional annotations were among the 25 categories.
Table 2.
Number of immunity-related genes in Tenebrio molitor transcriptome and Tribolium castaneum genome.
Table 3.
Statistics of DGE sequencing.
Figure 5.
Changes in gene expression profile between non-parasitized (NP) and parasitized (P) Tenebrio molitor pupae.
The number of up-regulated and down-regulated genes between NP and P groups is summarized here.
Figure 6.
Selected major enriched GO terms.
Transcripts were annotated in three categories: cellular components, molecular function, and biological processes.
Figure 7.
Verification of differentially expressed genes by qRT-PCR.
The relative expression levels of these unigenes indicate the log2 ratio of non-parasitized (NP) and parasitized (P) Tenebrio molitor pupae by DGE and qRT-PCR, respectively. Error bars indicate standard deviations of averages from three replicates.
Table 4.
A list of Tenebrio molitor immune-related genes that were differentially transcribed after parasitization by Scleroderma guani.