Figure 1.
Location of polyribosomes in dendrites of B2 barrel.
Electron micrographs of B2 barrel hollow showing polyribosomes (arrows) located in the dendritic spine and associated with excitatory synapse (A), located in the dendritic spine and associated with inhibitory synapse (B), located in the dendritic shaft and associated with excitatory synapse (C) and located in the dendritic shaft and associated with inhibitory synapse (D). Scale bar: 0.5 μm.
Figure 2.
Density of polyribosomes in dendritic spines.
Conditioning increases the density of polyribosomes located in spines and associated with excitatory (ANOVA, p<0.001) and inhibitory synapses (ANOVA, p<0.001). A. Total density of polyribosomes located in dendritic spines in: control, conditioned (CS+UCS) and pseudoconditioned (PSEUDO) group. B. Density of polyribosomes located in spines and associated with excitatory synapses. C. Density of polyribosomes located in spines and associated with inhibitory synapses. All graphs show means ± SD (**p<0.01, ***p<0.001).
Figure 3.
Density of polyribosomes in dendritic shafts.
Conditioning induces an increase in the density of polyribosomes associated with inhibitory synapses and located in dendritic shafts (ANOVA, p<0.001), while pseudoconditioning decreases the density of polyribosomes not associated with synapses and located in dendritic shafts (ANOVA, p<0.01). A. Density of polyribosomes associated with synapses and located in dendritic shafts in: control, conditioned (CS+UCS) and pseudoconditioned (PSEUDO) group. B. Density of polyribosomes associated with inhibitory synapses and located in dendritic shafts. C. Density of polyribosomes associated with excitatory synapses and located in dendritic shafts. D. Density of polyribosomes not associated with synapses and located in dendritic shafts. All graphs show means ± SD (**p<0.01, *** p<0.001).
Figure 4.
Location of polyribosomes in heads/necks/bases of dendritic spines.
Conditioning and pseudoconditioning do not change the location of polyribosomes in the dendritic spines. A. Electron micrograph of B2 barrel hollow showing polyribosomes (arrow) located in the head of single-synapse spine (s). B. Electron micrograph of B2 barrel hollow showing polyribosomes (arrows) located in the neck of single-synapse spine (s). Scale bar: 0.5 μm. C. Location of polyribosomes in dendritic spines in: control, conditioned (CS+UCS) and pseudoconditioned (PSEUDO) group. Graph shows means ± SD.
Figure 5.
A. Excitatory synapses located on spines containing polyribosomes have larger postsynaptic density area than those on spines without polyribosomes (two-way ANOVA, p<0.0001) and the synapses enlarge during conditioning (ANOVA, p<0.01). Synapses on spines without polyribosomes do not significantly differ in size in the control, in the conditioned group (CS+UCS) and in the pseudoconditioned group (PSEUDO). Graph shows means ± SD (*p<0.05, *p<0.01, ***p<0.001). B. Inhibitory synapses located on spines containing polyribosomes have larger postsynaptic density area of than those located on spines without polyribosomes (two-way ANOVA, p<0.0001) and the synapses enlarge during conditioning (ANOVA, p<0.05). Synapses on spines without polyribosomes do not significantly differ in size in the control, in the conditioned group (CS+UCS) and in the pseudoconditioned group (PSEUDO).