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Figure 1.

The different vitamin B6 vitamers and their intracellular conversions.

PK = pyridoxal kinase. PNPO = pyridox(am)ine phosphate oxidase. PLP-Ph = PLP-phosphatase.

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Figure 1 Expand

Figure 2.

Expression of the enzymes involved in vitamin B6 metabolism in human cell lines and tissue lysates.

Actin was used as a loading control (cell lysates only). Depicted are representations of at least duplicates. PK = pyridoxal kinase. PNPO = pyridox(am)ine phosphate oxidase. PLP-Ph = PLP-phosphatase. Small Int = small intestine. HepG2 = HepG2 cells. Caco-2 = Caco-2 cells.

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Figure 2 Expand

Table 1.

Apical and basolateral amounts of PN, PM and PL in time during incubation with 100 and 1000 nmol/L of either B6 vitamer.

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Figure 3.

Intracellular amounts of PLP during incubation with no B6 vitamers, PN, PM and PL.

Depicted are means ± SE of triplicates.

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Figure 4.

Changes in apical and basolateral amounts of PL during incubation with PN (A) or PM (B).

Depicted are means ± SE of triplicates.

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Figure 5.

Hypothesis of human intestinal vitamin B6 metabolism.

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