Figure 1.
Morphological comparison of adult plants, spikes, spikelets, and seeds from disomic addition line 59-11 and its parents, wheat cv. 7182 and Psathyrostachys huashanica. a
adult plants; b spikes; c spikelets and seeds. 1 7182, 2 Psathyrostachys huashanica, 3 59-11.
Table 1.
Morphological characteristics of 7182, Psathyrostachys huashanica, and the 6Ns addition line.
Figure 2.
Mitotic and meiotic patterns of the wheat-Psathyrostachys huashanica addition line 59-11.
a Somatic chromosomes in root tips, 2n = 44; b Chromosome behavior of PMCs at metaphase I, 2n = 22 II.
Table 2.
Chromosome pairing at metaphase I in PMCs from the disomic addition line 59-11 and its parents, wheat cv. 7182 and Psathyrostachys huashanica.
Figure 3.
Mitotic and meiotic GISH analysis of the wheat-Psathyrostachys huashanica addition line 59-11.
a Somatic metaphase of the disomic chromosome addition line with two Psathyrostachys huashanica chromosomes (yellowish-green color); b PMCs at meiotic metaphase I, where the yellowish-green color indicates a ring bivalent chromosome from Psathyrostachys huashanica (color figure online).
Figure 4.
Identification of Psathyrostachys huashanica chromosomes using EST-SSR and EST-STS markers in disomic addition line 59-11 and its parents, wheat cv. 7182 and Psathyrostachys huashanica.
a SWES2 and b CD452568 amplified specific bands in the addition line in chromosomes 6A, 6B, and 6DS, and, 6AL, 6BL, and 6DL, respectively (arrows). M marker, 1 Psathyrostachys huashanica, 2 7182, 3 chromosome addition line 59-11. Arrows indicate the diagnostic amplification products of Psathyrostachys huashanica.
Table 3.
Primers used to produce specific markers for chromosome 6Ns in Psathyrostachys huashanica.
Figure 5.
A-PAGE analysis of the disomic addition line and its parents, wheat cv. 7182 and Psathyrostachys huashanica.
1 7182, 2 Psathyrostachys huashanica, 3 59-11. The arrow shows specific bands from Psathyrostachys huashanica that appeared as α-gliadin in 59-11.