Figure 1.
Distribution of 4MTB-GSL contents in the two F2 populations obtained by crossing ‘TBS’ and ‘AZ26H’.
(A) F2 population grown in 2010, (B) F2 population grown in 2011.
Table 1.
SNPs between the parental lines identified by next generation sequencing.
Figure 2.
Linkage map of SNP markers showing polymorphism between ‘TBS’ and ‘AZ26H’.
Black bars and gray bars indicate QTL regions of 4MTB-GSL contents detected in 2010 and 2011, respectively.
Table 2.
QTL analysis of 4MTB-GSL contents in radish roots.
Table 3.
Comparison of 4MTB-GSL contents between different genotypes of SNP markers in GSL-QTL-3 and GSL-QTL-4.
Table 4.
Newly developed markers in QTL regions.
Table 5.
Sequence analysis of glucosinolate biosynthesis genes in R. sativus.
Figure 3.
Nucletide polymorphisms of RsMAM3, RsIPMDH1, and RsBCAT4 between ‘TBS’ and ‘AZ26H’.
The black and gray boxes indicate exons and introns, respectively. The black arrows show indels. The positions of dashed arrows indicate SNP sites and nucleotide variations. The numbers under the boxes indicate the start and stop sites of exons.
Figure 4.
RT-PCR analyis of candidate gene transcripts in ‘TBS’ and ‘AZ26H’.
RNAs were extracted from radish roots. Actin was used as a control to demonstrate equal RNA loading.
Figure 5.
Gene expression analyses of parental lines by real-time PCR.
(A) RsMAM3, (B) RsIPMDH1, (C) RsBCAT4. Gray and black bars indicate parental lines ‘TBS’ and ‘AZ26H’. Significant differences (P<0.01, LSD-t test) between the parental lines are indicated by asterisks.