Figure 1.
Neurons in hippocampus CA1 area.
(a) The picture showed the neurons in hippocampus CA1 area. The neurons in sham group (A) displayed regular appearance with large and round nuclei but pyknosis was observed in ischemia (C) and hypothermia group (B). (b) Compared with sham group(89.3±6.1) (A), the number of normal neuronal is fewer and the neurons of morphologic abnormality is more in ischemia group(47.3±4.5) (C). The number of survival neurons in hypothermia group(64.5±7.5) (B) is more than that in ischemia group. /400×visual field.
Figure 2.
Expression of GRP78 in hippocampus CA1.
(a) Immunohistochemistry showed the GRP78 was barely detected in sham group (A). The expression of GRP78 in hypothermia group (B) is much stronger than that in ischemia group (C) at reperfusion 24 hours. /400×visual field (b) Western blot analysis showed that the GRP78 was barely detected in sham group. In brains of ischemia group, it was increased 6 hour after 15 min of ischemia and gradually decreased thereafter; however, the degree of increase was much bigger in the hypothermia brains. (c) Quantitative analysis of Western blotting showed that hypothermia after ischemia significantly increased GRP78 after 15 minutes of ischemia (P<0.05 compared with ischemia brains at the same time points, 6 rats from each group at every time points were used for analysis).
Figure 3.
Expression of chop in hippocampus CA1.
(a) Immunohistochemistry showed the chop was barely detected in sham group (A).The expression of chop in hypothermia group (B) is much weaker than that in ischemia group (C) at reperfusion 24 hours. /400×visual field (b) Western blot analysis showed that the chop was barely detected in sham group. In brains of ischemia group, it was increased 6 hour after 15 minutes of ischemia and gradually decreased thereafter; however, the degree of increase was much smaller in the hypothermia brains. (c) Quantitative analysis of Western blotting showed that hypothermia after ischemia significantly decreased chop after 15 minutes of ischemia (P<0.05 compared with ischemia brains at the same time points. 6 rats from each group at every time points were used for analysis).
Figure 4.
Neuronal apoptosis in CA1 region of hippocampus induced by global cerebral ischemia.
Detection of apoptosis in hippocampus CA1 pyramidal neurons was carried out using Tunel staing. The sham group showed a large number of neurons and almost no TUNEL-positive cells (5.1±1.2) (A). In ischemia (C) and hypothermia (B) groups, the number of neurons were decreased and substantial TUNEL-positive cells were detected. The number of TUNEL-positive cells in hypothermia group (34.4±4.2) (B) is more than in ischemia group (40.5±5.7) (C), /400×visual field.