Figure 1.
Brain array coil (left) vs. cryogenic cooled resonator (CCR – right).
Upper panel: (b = 0) anatomical images (axial slice and coronal reconstruction) used for signal-to-noise ratio (SNR) estimation: ROI 1 (r = 20 voxels) is located in a region without signal in order to estimate the noise, ROI 2 (r = 5 mm) is located in the ventricles in order to estimate the signal intensity in a region with high (b = 0)-signal. Lower panel: Directional encoded color maps of FA (axial slice and coronal reconstruction) - directional information was incorporated by color coding the scalar FA map with the red, green and blue colors to label the left-right, ventral-dorsal, and caudal-rostral directions, respectively.
Figure 2.
Directional encoded color maps of FA overlaid into (b = 0) anatomical images: comparison of FA quality for scanning protocols (SPs) A (35 minutes, 1 average), B (110 minutes, 6 averages), and C (18 minutes, 1 average).
Coronal slices (upper panel) show only slight visual differences. The lower slice thickness of SP A (compared to SPs B and C) is visible in the reconstructed axial slices (lower panel). Directional information was incorporated by color coding the scalar FA map with the red, green and blue colors to label the left-right, ventral-dorsal, and caudal-rostral directions, respectively. FA-display threshold was 0.2.
Figure 3.
Location of ROIs for FA-value calculation in the septohippocampal nucleus (ROI I), in the corpus callosum (ROI II), and in the medial septal nucleus (ROI III).
Directional information was incorporated by color coding the scalar FA map with the red, green and blue colors to label the left-right, ventral-dorsal, and caudal-rostral directions, respectively. FA-display threshold was 0.2.
Figure 4.
FT results for seed points in the genu and along the corpus callosum, near the lateral septal nucleus, and in the olfactory path.
Left column: Location of the seed points. Columns 2–4: FT for mouse 1 with different scanning protocols (SP). Columns 5–6: FT for mice 2 and 3, respectively, with SP A (35 minute scan).
Figure 5.
Left panel – ROI analysis: FA-values (standard deviation as error bars) for different mice with different scanning protocols (SP) (colors red, green, blue represent scanning protocols A,B,C, respectively) and coefficients of variance (CV) averaged for 3 different ROIs in 3 different mice for the 3 scanning protocols. Right panel – TFAS: FA-values (standard deviation as error bars) for different mice with different scanning protocols (SP) (colors red, green, blue represent scanning protocols A,B,C, respectively) and coefficients of variance (CV) averaged for 3 different TFAS in 3 different mice for the 3 scanning protocols.