Figure 1.
Schematic diagram of the total BNP and proBNP assay systems.
BC203(Fab') is a common capture antibody in both systems. KY-BNP-II(Fab') is the detection antibody for the total BNP assay, and 18H5(Fab') is the detection antibody for the proBNP assay. ALP: Alkaline phosphatase; CDP-Star EmeraldII (Chemiluminescent Substrate): Disodium 2-chloro-5-(4-methoxy-spiro{1,2-dioxetane-3,2′-(5′-chloro)-tricyclo [3,3,1,13,7]decan}-4-yl)-1-phenyl phosphate.
Figure 2.
Standard curves for the proBNP (open circle) and total BNP (closed circle) assays (A). Plasma dilution curves (B).
Three plasma samples collected from three heart failure patients were serially diluted with buffer.
Table 1.
Recovery of standard glycosylated proBNP and BNP added to human plasma.
Table 2.
Effects of dilution on recovery rates with the proBNP and total BNP assay systems.
Table 3.
Intra- and Inter-assay precision of the proBNP assay systems.
Table 4.
Intra- and Inter-assay precision of the total BNP systems.
Table 5.
Cross-reactivity between proBNP and BNP.
Figure 3.
Gel filtration analysis of total BNP (A) and proBNP (B) in plasma from a heart failure patient.
Fractions were assayed using the total BNP (A) and proBNP (B) systems. The elution points for glycosylated proBNP, proBNP and BNP are indicated by red arrows. Black and red lines respectively show gel filtration analyses of total BNP (A) and proBNP (B) in the same plasma sample before and after deglycosylation.
Figure 4.
Plasma Levels of total BNP, proBNP, and NT-proBNP in different age groups.
Bar graph showing the total BNP, proBNP (A) and NT-proBNP levels (B). Values are means ± SE., *P<0.05 vs total BNP, proBNP, and NT-proBNP in 30∼39, †P<0.05 vs total BNP, proBNP, and NT-proBNP in 40∼49.
Figure 5.
The relationships between total BNP (A), proBNP (B), and NT-proBNP (C) and age.
Figure 6.
Plasma Levels of proBNP, total BNP, and NT-proBNP in normal and heart failure.
Bar graph showing the total BNP, proBNP (A) and NT-proBNP (B) levels in healthy subjects and heart failure patients with NYHA classes 1–2 and 3–4. *P<0.05 vs total BNP and proBNP in normal, †P<0.05 vs total BNP and proBNP in HF NYHA 1–2. Bar graph showing the total BNP, proBNP (C), proBNP/total BNP ratio (D) and NT-proBNP (E) levels in male and female in healthy subjects. Values are means ± SE. *P<0.05 vs male.