Table 1.
Composition of the experimental mice diets (g/kg).
Table 2.
Fatty acid composition of the experimental mice diets.
Figure 1.
Composition of salmon aqua feed changes n−3/n−6 PUFA ratio in mice consuming the salmon fillets.
Fish oil (FO) in aqua feed was partly replaced with rapeseed oil (RO), olive oil (OO) or soy bean oil (SO) and fed Atlantic salmon. The salmon fillets were used in Western diets (WDs) fed male C57BL/6J mice (n = 8/diet) for 10 weeks. Fatty acid composition was measured and the n−3/n−6 PUFA ratio calculated in (A) Atlantic salmon fillets (B) the WDs containing the fillets and (C) red blood cells (RBC) collected from mice consuming the WDs and reference diets. Data represent mean of duplicate measurements in A and B and mean+SEM (n = 5) in C. Asterisk(s) indicates significant different from FO-WD.
Table 3.
Fatty acid composition in RBCs from mice after consuming the experimental diets for 10 weeks.
Figure 2.
Composition of salmon aqua feed changes accumulation of POPs in mice consuming the salmon fillets.
Fish oil (FO) in aqua feed was partly replaced with rapeseed oil (RO), olive oil (OO) or soy bean oil (SO) and fed Atlantic salmon. The salmon fillets were used in Western diets (WDs) fed male C57BL/6J mice (n = 8/diet) for 10 weeks. Concentrations of 7PCBs and DDTs were measured in (A) Atlantic salmon fillets (B) the WDs containing the fillets and (C) epididymal white adipose tissue (eWAT) and (D) liver collected from mice consuming the WDs and a low fat reference diet. Data represent mean of duplicate measurements in A and B. Tissues from two animals were pooled to achieve sufficient material for POP analyzes, and data in C and D thus represent mean+SEM (n = 4). Asterisk(s) indicates significant different from WD-FO.
Table 4.
Body weight, energy intake, energy efficiency and apparent fat digestibility in mice fed the different diets for 10 weeks.
Table 5.
Organ weights (g) in the mice fed the experimental diets for 10 weeks.
Figure 3.
Fatty acid composition in salmon fillets influences development of insulin resistance in mice.
Male C57BL/6J mice (n = 8/diet) were fed WD-FO, WD-RO, WD-OO and WD-SO for 10 weeks. As references, two groups of mice received regular WD or LF diet. Plasma glucose (A) and insulin (B) were measured after overnight fasting. An intraperitoneal glucose tolerance test (GTT) was performed after 7 weeks of feeding (C) and an intraperitoneal insulin tolerance test (ITT) was performed after 8 weeks of feeding (D). Area under the curve (AUC) was calculated from the glucose tolerance (baseline was set to fasted blood glucose levels) (E) and insulin tolerance test (F). Data are presented as means+SEM (n = 8). *represents significant different from WD-FO (P<0.05). **represents significant different from WD-FO (P<0.01). ***represents significant different from WD-FO (P<0.005).
Figure 4.
Fatty acid composition in salmon fillets influences development of hepatic lipid accumulation in mice.
Male C57BL/6J mice (n = 8/diet) were fed WD-FO, WD-RO, WD-OO and WD-SO for 10 weeks. As references, two groups of mice received standard WD or LF diet. Lipids were extracted from liver and (A) total lipids; (B) triacylglycerol (TAG); (C) free cholesterol, and (D) steryl ester (SE) were quantified. Data are presented as means+SEM (n = 8). Asterisk(s) indicates significant different from WD-FO.