Figure 1.
The taxonomic distribution analysis of M. roseus proteome.
Figure 2.
Maximum likelihood tree based on the concatenation of 39 ribosomal proteins.
The use of the neighbour-joining method resulted in a similar tree topology. The ribosomal proteins from Aquifex aeolicus VF5 were used as an outgroup. Numbers at nodes represent bootstrap values (100 replications of the original dataset), only numbers above 50% are shown. The scale bar represents the average number of substitutions per site.
Figure 3.
Carbohydrate-active enzymes containing CBM domains.
Abbreviations: PL, polysaccharide lyase; CBM, carbohydrate binding module; Por, Por secretion system C-terminal sorting domain; FN3, fibrinonectin type 3 domain; GH, glycoside hydrolase; SP, signal peptide.
Figure 4.
Zymographic analysis of hydrolytic activities against carboxymethyl cellulose.
1 - Proteins washed from the surface of aerobically grown cells with 1 M NaCl; 2 - Proteins washed from the surface of aerobically grown cells with 1% SDS; 3 - Proteins washed from the surface of anaerobically grown cells with 9 M urea; 4 - Proteins washed from the surface of aerobically grown cells with 9 M urea. Positions of molecular weight markers (sizes are shown in kD) are indicated by arrows. Note that the intracellular protein fraction and culture supernatant showed no activity against CMC on zymograms.
Table 1.
Endoglucanases predicted from the M. roseus genome.
Figure 5.
An overview of the metabolism of M. roseus.
Enzymes and proteins identified in the genome are in blue, energy-rich intermediate compounds are in red. Enzyme abbreviations: HK, hexokinase; PGI, phosphoglucose isomerase; PFK, phosphofructokinase; FBA, fructose-bisphosphate aldolase; TIM, triose phosphate isomerase; GPDH, glyceraldehyde 3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; PGM, phosphoglycerate mutase; PYK, pyruvate kinase; LDH, lactate dehydrogenase; PDH, pyruvate dehydrogenase; POR, pyruvate ferredoxin oxidoreductase; ACS, acetyl-CoA synthetase; Pyc, pyruvate carboxylase; Mae, malic enzyme; Pck, phosphoenolpyruvate carboxykinase; GltA, citrate synthase; Acn, aconitase; Icd, isocitrate dehydrogenase; Oor, 2-oxoglutarate ferredoxin oxidoreductase; Fum, fumarase; Mdh, malate dehydrogenase; SDH, succinate dehydrogenase; Tkt, transketolase; TalB, transaldolase; ACIII, alternative complex III; COX, cytochrome c oxidase; ArsC, arsenite reductase; NAD(P) trans HDR, NAD(P) transhydrogenase; Nos, nitrous oxide reductase; PPase, pyrophosphatase. Other abbreviations: GAP, glyceraldehyde-3-phosphate; 3-PG, 3-phosphoglycerate; 2-PG, 2-phosphoglycerate; L-Ru-5-P, L-ribulose-5-phosphate; D-Ru-5-P, D-ribulose-5-phosphate; Fdox/Fdred, ferredoxin, oxidized and reduced form; Cyt, cytochrome; Pi, phosphate; PPi, pyrophosphate; CoA, coenzyme A.