Figure 1.
Straighthead phenotypes in parents of two mapping populations.
Resistant parents Zhe733 and Jing185 had fully developed panicles while susceptible parents R312 and Cocodrie had panicles with severely distorted spikelets.
Figure 2.
Distribution of RILs of two populations.
Distribution of straighthead ratings among 170 Zhe733/R312 F9 RILs and two parents Zhe733 and R312 (A), and among 91 Cocodrie/Jing185 F9 RILs and two parents Cocodrie and Jing185 (B). Coefficient of variation was 16.7% and 13.1% in Zhe733/R312 and Cocodrie/Jing185 populations, respectively.
Figure 3.
Linkage map constructed by polymorphic SSR markers in Zhe733/R312 population (A), and Cocodrie/Jing185 population (B).
Number 1 to 12 represent chromosome number in rice genome.
Figure 4.
Straighthead-associated QTLs identified in Zhe733/R312 population (A), and Cocodrie/Jing185 population (B).
Black bar shows the position of each QTL on rice chromosome (Chr).
Table 1.
QTLs identified in two recombined inbred line (RIL) F9 populations using Qgene 4.3.8.
Figure 5.
Fine mapping of qSH-8 on chromosome 8 using Zhe733/R312 (A-C) and Cocodrie/Jing185 (D-F) F9 RIL populations.
Genetic distance cM is the number between markers and physical distance is placed below markers, qSH-8 in a 1.2 cM region between RM6838 and RM72 (A), a 340 kb region between RM22573 and RM22589 (B) and a 290 kb region between RM22573 and InDel 27 (C) in Zhe733/R312; and a 2.8 cM between RM22559 and RM72 (D), a 710 kb region between AP3858-1 and RM22613 (E), and a 690 kb region between InDel 11 and RM22613 (F) in Cocodrie/Jing185.
Table 2.
Recombinants identified in the target region from population Zhe733/R312 between RM6838 and RM72.
Table 3.
Recombinants identified in the target region from population Cocodrie/Jing185 between RM22559 and RM72.
Table 4.
Polymorphic SSR markers designed for fine mapping straighthead resistance QTL qSH-8 on chromosome (chr) 8 using two recombined inbred line (RIL) F9 populations.
Table 5.
Polymorphic InDel markers for fine mapping QTL qSH-8 on chromosome (chr) 8 using Zhe733/R312 recombined inbred line (RIL) F9 population.
Table 6.
Association of InDel 11 genotype with straighthead phenotype in a global germplasm collection.
Table 7.
Association of markers with straighthead phenotype using two recombined inbred line (RIL) F9 populations and a global germplasm collection including 72 accessions (some germplasm accessions had no parental alleles for a certain marker).