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Figure 1.

Map of five T. inunguis sampled rivers.

(I) Colombian Amazon, (II) Peruvian Amazon, (III) Ucayali, (IV) Marañón, and (V) Napo.

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Figure 2.

Map of six T. manatus sampled rivers.

(I) Sinú, (II) Northern Magdalena, (III) San Jorge, (IV) Magdalena’s Ciénaga de Paredes, (V) Meta, and (VI) Orinoco.

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Figure 3.

T. inunguis Median Joining network (MJN).

a) 361bp MJN of Colombian, Peruvian populations, and previously published Brazilian populations [24]. b) 410bp MJN of Colombian and Peruvian manatees. Both MJN revealed one D-loop haplotypes cluster for Amazonian manatees (see Figure 1 for Colombian and Peruvian population name abbreviations; Brazilian populations are abbreviated following Cantanhede et al. 2005∶1-Japurá, 2-Tefé, 3-Negro, 4-Solimões, 5-Amazonas, 6-Pará)). Haplotypes names are written outside of haplotypes pie charts, while population names are written inside.

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Table 1.

D-loop number of haplotypes (h), nucleotide (π), and haplotype (Hd) diversity indices with 95% confidence intervals, and 15 microsatellite loci estimates of number of alleles per locus (NA), mean observed heterozygosity (HO), and mean expected heterozygosity (HE), for T. inunguis, and T. manatus populations.

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Figure 4.

T. manatus Median Joining network (MJN).

The MJN reveals two D-loop haplotypes clusters of Colombian manatees (see Figure 2 for population name abbreviations). Haplotypes names are written outside of haplotypes pie charts, while population names are written.

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Table 2.

Pairwise FST and P values estimates for five T. inunguis populations.

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Table 3.

Pairwise FST and P values estimates for six T. manatus populations.

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Table 4.

Migration models for T. inunguis and T. manatus ranked according to the harmonic mean of the likelihood, and Bayes factors for every model vs. that with the highest likelihood.

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