Figure 1.
Natriuretic Peptide Structures.
Amino acid sequence of mature C-type natriuretic peptide (CNP), Dendroaspis natriuretic peptide (DNP) and the designer natriuretic peptide, CD-NP.
Figure 2.
Experimental protocol of CD-NP (170 ng/kg/min in 5% glucose solution) or Vehicle (5% glucose solution) delivery in Sham or UNX-operated male Wistar rats by subcutaneous (SubQ) osmotic mini-pump.
Figure 3.
Natriuretic Peptide Generation of cGMP.
In vitro 3′,5′-cyclic guanosine monophosphate (cGMP) generation in human embryonic kidney 293 cells stably transfected with either the GC-A (upper graph) or GC-B (lower graph) receptor in response to a 10−6 M dose of CNP (yellow bar), DNP (orange bar) and CD-NP (green bar) compared with no treatment. Values are mean ± SEM. *P<0.05 vs no treatment.
Table 1.
Cardiac Structure, Systolic Function and Blood Pressure.
Figure 4.
Effect of CD-NP treatment on preserving diastolic function in UNX+CD-NP rats compared to UNX+Vehicle rats. Values are mean ± SEM. *P<0.05 vs Sham+Vehicle and P<0.05 vs UNX+Vehicle.
Figure 5.
Representative histology images at 10x objective magnification of the LV stained with picrosirius red (upper panels) and the quantification of LV fibrosis staining (lower graph) in Sham+Vehicle, UNX+Vehicle and UNX+CD-NP rats. Values are mean ± SEM. *P<0.05 vs Sham+Vehicle and P<0.05 vs UNX+Vehicle.
Figure 6.
. Effect of CD-NP treatment in suppressing plasma aldosterone in UNX+CD-NP rats compared to UNX+Vehicle rats. Values are mean ± SEM. *P<0.05 vs Sham+Vehicle and P<0.05 vs UNX+Vehicle.