Table 1.
List of bacterial strains, plasmids, and bacteriophages.
Figure 1.
Plasmid profile of donor L. lactis DGCC7167 + pGh9::ISS1, phage resistant transconjugants K (derived from recipient L. lactis 1403S) and KLM (derived from recipient LM2345).
Figure 2.
pKLM map and plasmid comparison.
Rings from inside out: pKLM ORFs; pKLM GC content; nucleotide identity with pLN; nucleotide identity with pMRC01.
Table 2.
List and nucleotide sequence of pKLM/pLN CRISPR spacers.
Figure 3.
Expanded view of the CRISPR/Cas locus of pKLM/pLN, pF8E, pG6, and pG6::pRS4R.
View of cas genes and CRISPR arrays correlated to phage resistance phenotype. pKLM/pLN contains the full spacer array which gives resistance to all 4 listed phages. pF8E contains an insertion within the array which disrupts spacers s1-s9 as well as resistance to phage encoded by those spacers. pG6 contains a deletion of spacers s2-s9, and resistance to phage encoded by those spacers is lost. pG6::pRS4R contains an engineered s4 which provides resistance to all 3 phages it has identity to.
Table 3.
Escape phage analysis.