Figure 1.
srf6 SALK T-DNA triggered suppression of cobra phenotype and inheritance of epicob-6.
(A) Quantification of hypocotyl length in four-day-old dark grown seedlings. Genotypes, mean and SE are indicated, n = 30–40. (B) Four-day-old dark grown seedlings of Col-0, srf6-1, srf6-1cob-6, epicob-6 and cob-6. (C) Phenotype comparison of etiolated epicob-6 for four generations.
Figure 2.
COBRA transcript level increase in the suppressed cobra.
(A) qPCR determined COBRA transcript level in etiolated seedlings grown on ½ MS medium. Genotypes, mean and SE are indicated, n = 3 pools of seedlings. Asterisks indicate P values for comparison with cob-6: * P<0.05; ** P<0.001 (Student’s t-test). (B) Phenotype comparison of etiolated srf6-1cob-6 and srf6-1cob-4 seedlings. Pictures are representative of multiple plants for each genotype.
Table 1.
Hypocotyl length of the randomly selected SALK T-DNA r1, r2 and r3cob-6 and the srf4-1 SAIL T-DNA cob-6 double mutants.
Figure 3.
Transmission of epicob-6 phenotype.
Four-day-old etiolated seedlings in the F2 progeny from a cross between epicob-6 and cob-6. Genotypes are indicated. Pictures are representative of multiple plants for each genotype.
Figure 4.
The effect of epigenome modification on srf6 SALK T-DNA caused cob-6 suppression.
Etiolated seedlings grown on ½ MS medium with (A) 30 µM of DNA methylation inhibitor 5-Azacytidine (5Aza) (B) 30 µM of DNA methylation inhibitor zebularine (Zeb). (C) 1.6 µM of histone deacetylase inhibitor Trichostatin A (TSA). Genotypes are indicated. Pictures are representative of multiple plants for each genotype. (D) qPCR determined COBRA transcript level in etiolated seedlings grown on ½ MS medium with 30 µM 5-azacytidine (5-Aza) normalised against Col-0 without 5-Aza. Genotypes, mean and SE are indicated, n = 3 pools of seedlings. (E) The effect of DNA methylation mutants on srf6 SALK T-DNA caused cob-6 suppression. Genotypes are indicated. Pictures are representative of multiple plants for each genotype.
Table 2.
Effect of the different DNA methylation mutants on the trans cob-6 SALK T-DNA interaction.