Table 1.
Urine fibronectin co-purified proteins identified by mass spectrometry.
Figure 1.
Quantification of signal intensities of co-purified candidate proteins of healthy and FIC diseased cases.
Western blot signal intensity of healthy controls (white columns, left, n = 20) and diseased urine samples (grey columns, right, n = 16) were compared for the following co-purified proteins: Complement component 4a (C4a) (A), galectin-7 (B), fatty acid-binding protein, intestinal (I-FABP) (C), thioredoxin (D), NF-κB p65 (E) and p38 MAPK (F). The according Western blot strips visualize the quantitative difference of signal intensities. The left strips show representative control blots, the right ones blots with FIC urine. The corresponding band sizes are displayed in black boxes. Signals were quantified by densitometry and statistical significance was calculated using the Mann-Whitney test. Data are represented in a column bar graph as means with SEM. Expression level of C4a (A) shows a significant (***p≤0.001) increase in urine of FIC diseased cases with an almost 5-fold higher concentration compared to urine of healthy controls. Galectin-7 (B) is significantly (*p≤0.05) upregulated in FIC diseased samples compared to healthy control samples with a 45-fold higher expression in FIC affected cases. Quantification of I-FABP expression (C) results in a significant (*p≤0.05) decrease in urine of FIC cases compared to urine of healthy controls. Thioredoxin (D) is significantly (***p≤0.001) increased by a factor of 7.5 in FIC compared to urine of controls. Abundances of NF-kB p65 (E) significantly (***p≤0.001) increase in diseased specimens compared to healthy specimens with a 5-fold higher expression just as p38 MAPK (F) showing an 6.5-fold higher expression (*p≤0.05) in FIC diseased urines in comparison to healthy control urines.
Figure 2.
H&E staining of normal feline bladder (A) and FIC diseased bladder (B).
Histological sections stained with Haematoxylin and Eosin. Healthy urinary bladder section (A) shows characteristic architecture compared with FIC diseased bladder tissue (B), where a loss of normal bladder wall physiology can be seen. Notice the marked loss of transitional cell epithelium, the intramucosal bleeding and oedema in the FIC section (B). a = Transitional cell epithelium, b = Lamina propria mucosae, c = Loss of transitional cell epithelium, d = Intramucosal bleeding and oedema.
Figure 3.
Expression of co-purified proteins in healthy bladder tissue.
Immunohistochemical double labelling of CD117 and co-purified proteins in a representative healthy bladder tissue. DIC image of healthy bladder tissue (A). CD117 (green) shows a marked reactivity in the epithelial cells of the urothel and in the interstitial cells of the lamina propria of the healthy bladder (B). Overlay image of C4a (red) and CD117 (green) reveals considerable co-localization (overlapping results in yellow colour) at the cell nuclei of the urothelial cells and a scattered expression in the interstitial cells of the lamina propria (C). Galectin-7 (red) and CD117 (green) show a co-localization in the umbrella cells (marked with an asterisk) of the transitional cell epithelium, whereas reactivity of both proteins in the epithelial cells of the urothel indicate a co-expression. Cells of the lamina propria are only CD117 positive (D). I-FABP (red) and CD117 (green) overlay is visible only in the interstitial cells of the lamina propria. Additionally, I-FABP reactivity is seen extracellularly and is distinctly expressed in the basal membrane (E). Thioredoxin (red) and CD117 (green) co-localize distinctly at all cell nuclei of the transitional epithelial cells and in the interstitial cells of the subepithelial tunic (F). The blue colour reveals staining of cell nuclei (DAPI). a = Transitional cell epithelium, b = Lamina propria mucosae, c = Inserted box shows magnification of respective cells in the lamina propria mucosae.
Figure 4.
Expression pattern of fibronectin and its co-purified proteins in healthy and diseased bladder tissue.
Urinary bladder expression of fibronectin (green) and its co-purified proteins (red) in a representative healthy (left panels) and FIC diseased bladder (right panels). Physiological distribution of fibronectin (green) in healthy bladder (A). Extracellular matrix of the lamina propria mucosae and the muscle tunic show a distinct immunoreactivity for fibronectin, whereas a loss in FIC affected bladder tissue (B), especially in the subepithelial and muscular tunices, is evident. C4a (red) is moderately expressed in the apical transitional cell epithelium of the physiological bladder (C) and disappears in the FIC affected bladder tissue (D). Galectin-7 (red) is expressed especially in umbrella cells of the transitional epithelium and around blood vessels in the lamina propria under normal condition (E). In contrast, expression changes profoundly in FIC affected bladders to distinct expression in the transitional cell epithelium (F). Reactivity of I-FABP (red) throughout all tunices in healthy bladder (G) almost disappears in FIC diseased tissue (H). Thioredoxin (red) reveals a predominant signal in the entire healthy bladder tissue (I) compared to a leakage of thioredoxin into the lumen (arrow) of FIC affected bladder tissue resulting in a slight immunoreactivity of the diseased bladder tissue (J). The blue colour reveals staining of cell nuclei (DAPI). a = Transitional cell epithelium, b = Lamina propria mucosae, c = Muscle tunic, d = Normal vessel.
Figure 5.
Immunohistochemical double labelling of candidates in healthy (left panels) and diseased bladder tissue (right panels).
Immunohistochemical double staining of a healthy urinary bladder (A) shows considerable colocalization of fibronectin (green) and its interactor thioredoxin (red) in the subepithelial and muscular tunices. In contrast, lack of green and red colour is evident in FIC (B), indicating a loss of both fibronectin and thioredoxin from its normal distribution in healthy bladder tissue. Overlay image of thioredoxin (red) and NF-κB p65 (green) in a healthy bladder tissue (C) shows a predominant signal of thioredoxin in all tunices of the bladder, whereas NF-κB p65 is not detectable in any tunic of the healthy bladder. In contrast, thioredoxin and NF-κB p65 colocalize (overlapping results in yellow colour) in the lamina propria mucosae with the highest expression in the extracellular matrix of FIC diseased bladder tissue (D). P38 MAPK signal is of moderate intensity localized in transitional epithelium cells and around few blood vessels in the healthy bladder tissue (E). Note that the signal is exclusively of yellow colour indicating a colocalization with thioredoxin, whereas a green colour signal is not visible at all. In contrast, p38 MAPK (green) was highly expressed in the cytoplasm of umbrella cells of the transitional cell epithelium as well as a scattered expression around cell nuclei in the subepithelial and muscular tunices of FIC diseased bladder sections without distinct colocalization (F). The blue colour reveals staining of cell nuclei (DAPI). a = Transitional cell epithelium, b = Lamina propria mucosae, c = Muscle tunic.