Figure 1.
Temperature-tolerant-fast-COLD-PCR in emulsion: Overview of the steps involved.
Multiplex pre-amplification from genomic DNA; emulsification with gene-specific primers; mixing into a single tube; and temperature-tolerant emulsion-based fast-COLD-PCR.
Table 1.
Cell lines used in the present study.
Table 2.
Primer sequences used in this study.
Table 3.
PCR thermocycling conditions utilized in the present work.
Figure 2.
Temperature-tolerant COLD-PCR in emulsion, TT-fast-eCOLD-PCR: Enrichment of mutations in multiple DNA sequences in a single tube.
A 5% mutation abundance was evaluated for TP53 gene exons 6–9 by conventional PCR (left panels) and TT-fast-eCOLD-PCR (right panels). Duplicate experiments are depicted in each case. The enrichment of the mutations in all four exons is estimated from the chromatograms.