Table 1.
Patient characteristics.
Figure 1.
Dengue virus in plasma and peripheral blood mononuclear cells.
Comparisons of dengue viral (DENV) RNA levels in plasma and PBMC subpopulations in DF and DHF cases two days prior to defervescence (fever day −2) (A). * indicates statistically significant differences in DENV RNA levels between DF and DHF (P<.05). +indicates differences between DENV RNA levels in CD20+ cells compared to CD14+ and CD2+ cells (P<.05). (B, C, D, E) Levels of DENV RNA levels in plasma (B), CD14+ (C), CD2+ (D) and CD20+ (E) cells on fever day −2 and fever day −1. Each line represents individual cases: solid lines: DHF, dashed lines: DF.
Figure 2.
DENV RNA levels in plasma and PBMC subpopulations in primary and secondary infections.
Plasma and PBMC cell subpopulations of 11 primary and 15 secondary DENV infections, all of whom had DF, were analyzed. * indicates statistically significant differences in DENV RNA levels between primary and secondary infection (P<.05). +indicates differences between DENV RNA levels in CD20+ cells compared to CD14+ and CD2+ cells (P<.05).
Figure 3.
Positive and negative strand DENV RNA levels in unfractionated PBMC.
Quantitative PCR for positive and negative strand viral RNA was performed with RNA extracted from unfractionated PBMC collected on fever day-2 from subjects infected with DENV1 (A), DENV2 (B), DENV3 (C), or DENV4 (D). The range of the positive strand DENV RNA was 0 to 6069 copies/million cells. Negative strand DENV RNA was detected in 6 of 18 samples with detectable positive strand RNA and the levels ranged from 5 to 35 copies/million cells.
Table 2.
Univariate correlations between indicators of disease severity and viral RNA levels in plasma and cell fractions collected on fever day-2.