Figure 1.
Developmental stages of the abalone H. diversicolor that were sampled for transcriptomic analyses.
The stages include two-cell (2CELL, 0.5 hpf), morula (MORU, 3.2 hpf), trochophore (TROC, 9.5 hpf), 19 hpf veliger (19VEL), 58 hpf veliger (58VEL), late competent veliger larvae (74VEL, 74 hpf), postlarvae after 3 days settlement (144PL, 144 hpf) and adult intestinal tissue (INTE, 18 months). Scale bar = 100 µm.
Figure 2.
Overview of H. diversicolor transcriptome sequencing and assembly.
(A) Size distribution of the unigenes. (B) Coverage of the assembled sequences with length distributions. The red plots represent those assembled sequences without BsgI restriction sites at their 5' or 3' ends, and the blue plots represent those assembled sequences with BsgI restriction sites at their 5' or 3' ends, which explains the short contigs/scaffolds with high coverages.
Table 1.
Summary of the sequencing and assembly statistics.
Figure 3.
Taxonomic distribution of best match sequences with BLAST alignments to the H. diversicolor unigenes (E ≤1e−5).
Figure 4.
Gene ontology (GO) annotation of the H. diversicolor larval transcriptome.
The top 10 gene ontology (GO) categories under Biological process, Cellular component and Molecular function for the early developmental transcriptomes of H. diversicolor.
Table 2.
The top 20 highly expressed sequences with their associated BLAST matches (E-value<1e−5).
Figure 5.
Validation of 20 temporal dynamics by qPCR.
For the qPCR data, the lowest expression level of each gene was set as 1, and the other expression levels are indicated as the fold-change relative to it. The 454 expression levels of each gene were normalized to the 454 data for OAZ1/YB1 and rescaled to the qPCR scales. Abbreviation: r, Pearson’s correlation coefficient.
Figure 6.
Clusters of the expression levels of the candidate genes.
Dendrograms of the gene expression patterns for the 636 genes assigned 2 or more stars are shown. The clustering indicates similar expression patterns among the developmental stages (x-axis) and among the genes (y-axis). The bar color reflects the gene expression level from green (low) to black (medium) to red (high). The partial gene names used for the analysis, whose expression levels were extremely high during the two-cell, trochophore and 144 postlarval stages, are displayed in the chart. The gene IDs in these three clusters are listed in Table S3.