Figure 1.
Red and green represent up-regulation and down-regulation, respectively. Lymph node metastatic samples are indicated by blue lines and primary tumors indicated by black lines.
Table 1.
Genes up-regulated in lymph node metastasis compared to primary tumor.
Figure 2.
Heat map view of the PRC2 network gene expressions in primary tumor and lymph node metastasis.
Figure 3.
Validation of EZH2 and EED mRNA expressions by real-time PCR.
White bars indicate primary tumor and the adjacent black bars indicate their matched lymph node metastasis.
Figure 4.
Validation of EZH2 protein expression by quantitative image analysis of whole tissue sections.
A: Proportion of EZH2 expressing tumor cells in paired lymph node and primary tumor sections are presented as adjacent bars. Percentage of EZH2+ tumor cells among the total number of tumor cells are indicated by Y axis. B: Correlation between percentage of EZH2 expressing tumor cells and percentage of proliferating cells. Lymph node metastases are indicated by solid circle and primary tumors by open circle. The Pearson correlation coefficient is 0.74 with p-value 0.001.
Figure 5.
Immunohistochemical staining and quantitative image analysis of whole tissue sections of E-cadherin protein expression.
Proportion of E-cadherin expressing tumor cells in paired lymph node and primary tumor sections are presented as adjacent bars. Percentages of E-cadherin+ tumor cells among the total number of tumor cells are indicated by Y axis.