Figure 1.
Temperature profiles of the thermal treatments to which Acropora millepora fragments were exposed.
Non-preconditioned (NPC) treatment with no pre-stress period prior to exposure to 31°C. Pulse –1 (P1) treament was exposed to a 2-day 28°C pre-stress and returned to ambient temperature for 1 week prior to thermal challenge. Sustained –1 (S1) treatment was exposed to 10 days of 28°C prestress. Sustained –2 treatment was exposed to 14 days of 28°C prestress. Pulse –2 (P2) treament was exposed to a 2-day 28°C pre-stress and returned to ambient temperature for 2 weeks prior to thermal challenge. Sustained –2 treatment was exposed to 14 days of 28°C prestress. Ambient control (C) treatment was not challenged with any increase in temperature. This figure expands upon a smaller dataset originally published by Bellantuono et al. [30].
Figure 2.
Symbiodinium density (algal cells per square centimeter).
Resident Symbiodinium densities at 6 sampling times throughout the course of the experiment; days 23, 27, and 29 represent 2, 6, and 8 days of exposure to 31°C. Asterisks indicate group is significantly different from controls (p<0.001, one-way ANOVA with Tukey HSD, n = 4). A portion of the data presented in this figure was analyzed previously by Bellantuono et al. [30].
Figure 3.
Rows A, B, and C represent gene expression from NPC, S1, and C treatments following 2, 4, and 8 days of 31°C, respectively. Left column contains principal component analysis plots of differentially expressed genes. Center column pie chart illustrated the number and trend of differentially expressed genes, with overlaps indicating differentially expressed genes detected across treatments. Column right indicates fold change differences between shared differentially expressed genes NPC and S1 treatments, both in reference to control.
Table 1.
Differentially expressed genes at two, four, and eight days of thermal challenge.