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Figure 1.

Map of study zones in Central Chile. a) Enlargement of study zones. b) Photo of the outfall.

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Figure 2.

Diagram of algal thallus parts of Lessonia trabeculata sampled for metal analyses.

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Table 1.

Metal measures in certified material BCSS-1 (marine sediment) and BCR-279 (sea lettuce, Ulva lactuca). Confidence intervals at 95% (n = 3).

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Table 2.

Compensation depths and bathymetry in sampling stations from the outfall and control zones.

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Table 3.

Metal concentrations in sediments and measured in the outfall wastewaters.

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Table 4.

Multivariate analysis of variance (MANOVA) at 95% confidence showing interactions between main factors (zones, sampling stations, and algal parts) and 9 dependent variables assessed (metals).

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Figure 3.

Metal concentrations in different thallus parts of L. trabeculata collected from different sampling stations.

Sampling stations located at 5,30 and 60 m from the sewage pipe at the outfall zone, named 1V, 2V, and 3V, respectively; Same sampling design at control zone, sediment samples taken at 5, 30, and 60 m from the shore (reference point, no outfall), named 1F, 2F, and 3F, respectively. Sampling carried out during austral summer (January) of 2009. Intervals at 95% confidence (n = 3).

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Figure 4.

Principal component analysis (PCA) of metals measured in blades, stipes and holdfasts of L. trabeculata from the different sampling stations.

Sampling stations located at 5, 30 and 60 m from the sewage pipe at the outfall zone, named 1V, 2V, and 3V, respectively; Same sampling design at control zone, sediment samples taken at 5, 30, and 60 m from the shore (reference point, no outfall), named 1F, 2F, and 3F, respectively.

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Table 5.

Tendencies from MANOVA and posteriori Tukey test.

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Table 6.

Correlations (R values) of metal concentrations in sediments and thallus parts of L. trabeculata.

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