Figure 1.
Y2H between FBX and ASK proteins.
(A) Number of FBX proteins that interacted with each ASK protein. (B) Phylogeny of ASK proteins using the unweighted pair group method with arithmetic mean of Clustal W (http://www.genome.jp/tools/clustalw/). ASK proteins, which interacted with more than 40 FBX proteins, are showed in red.
Figure 2.
Relationship between variable domains of FBX proteins and ASK proteins by Y2H.
Total numbers of FBX proteins carrying each domain (FBA, Kelch, LRR, FBD, DUF295, TUB and others) or none are indicated in parenthesis. Note that the number includes overlap of FBX proteins carrying multiple domains.
Figure 3.
Interaction of FBX and ASK proteins in vivo.
BiFC analyses were performed using combinations of four FBX (At1g30890, At3g03360, At3g04660 and At5g21040) (At1g30790, At3g03360, At3g04660 and At5g21040) and ASK proteins. (A) ASK1; (B) ASK2; (C) ASK3; (D) ASK4; (E) ASK5; (F) ASK7; (G) ASK8; (H) ASK10; (I) ASK11; (J) ASK13; (K) ASK14; (L) ASK16; (M) ASK17; (N) ASK18; (O) ASK19; (P) ASK20A; (Q) ASK20B.
Table 1.
Comparison between in vitro and in vivo interaction of randomly chosen FBXs and ASKs.
Figure 4.
Comparison of gene expression of ASK and FBX in various tissues using microarray data of GENEVESTIGATOR.
(A) Numbers of tissues where expression of ASK genes was observed were counted based on their expression profiles in Figure S1. (B) Numbers of ASK genes whose expression was observed in each tissue were counted based on their expression profiles in Figure S1. Numbers correspond to the tissue; callus (1), cell culture/primary cell (2), sperm cell (3), protoplast (4), guard cell protoplast (5), mesophyll cell protoplast (6), root protoplast (7), root cap protoplast (8), columella protoplast (9), lateral root cap protoplast (10), root epidermis and lateral root cap protoplast (11), root cortex protoplast (12), root endodermis and quiescent center protoplast (13), root stele protoplast (14), root phloem protoplast (15), root xylem protoplast (16), root cortex, endodermis and quiescent center protoplast (17), root epidermis protoplast (18), root epidermal atrichoblast protoplast (19), root culture (20), seedling (21), cotyledon (22), hypocotyl (23), radicle (24), imbibed seed (25), shoot apical meristem (26), inflorescence (27), flower (28), pistil (29), carpel (39), ovary (31), ovule (32), stigma (33), petal (34), sepal (35), stamen (36), anther (37), pollen (38), abscission zone (39), pedical (40), silique (41), replum (42), seed (43), embryo (44), endosperm (45), micropylar endosperm (46), peripheral endosperm (47), chalazal endosperm (48), testa (49), general seed coat (50), chalazal seed coat (51), suspensor (52), stem (53), developing meristemoid zone (54), node (55), shoot apex (56), cauline leaf (57), rosette (58), juvenile leaf (59), adult leaf (60), petiole (61), senescent leaf (62), hypocotyl (63), xylem (64), cork (65), leaf primordial (66), stem (67), axillary bud (68), axillary shoot (69), shoot apex (70), roots (71), primary root (72), root tip (73), meristematic zone (74), elongation zone (75), root hair zone (76), stele (77), pericycle (78) or lateral root (79). (C) Numbers of FBX genes whose expression was counted in each tissue based on their expression profiles in Figure S2. Numbers correspond to each tissue described in (B).
Figure 5.
Comparison of expression patterns of FBX and ASK genes in various tissues using microarray data of GENEVESTIGATOR.
Columns showing co-expression patterns between FBX and ASK genes are indicated by comparing the microarray data from Figures S1 and S2. Numbers correspond to the tissue; callus (1), cell culture/primary cell (2), sperm cell (3), protoplast (4), guard cell protoplast (5), mesophyll cell protoplast (6), root protoplast (7), root cap protoplast (8), columella protoplast (9), lateral root cap protoplast (10), root epidermis and lateral root cap protoplast (11), root cortex protoplast (12), root endodermis and quiescent center protoplast (13), root stele protoplast (14), root phloem protoplast (15), root xylem protoplast (16), root cortex, endodermis and quiescent center protoplast (17), root epidermis protoplast (18), root epidermal atrichoblast protoplast (19), root culture (20), seedling (21), cotyledon (22), hypocotyl (23), radicle (24), imbibed seed (25), shoot apical meristem (26), inflorescence (27), flower (28), pistil (29), carpel (39), ovary (31), ovule (32), stigma (33), petal (34), sepal (35), stamen (36), anther (37), pollen (38), abscission zone (39), pedical (40), silique (41), replum (42), seed (43), embryo (44), endosperm (45), micropylar endosperm (46), peripheral endosperm (47), chalazal endosperm (48), testa (49), general seed coat (50), chalazal seed coat (51), suspensor (52), stem (53), developing meristemoid zone (54), node (55), shoot apex (56), cauline leaf (57), rosette (58), juvenile leaf (59), adult leaf (60), petiole (61), senescent leaf (62), hypocotyl (63), xylem (64), cork (65), leaf primordial (66), stem (67), axillary bud (68), axillary shoot (69), shoot apex (70), root (71), primary root (72), root tip (73), meristematic zone (74), elongation zone (75), root hair zone (76), stele (77), pericycle (78) or lateral root (79). Percentage expression potential is shown from 0 (white) to 100 (dark brown) % according to the six-grade system.
Figure 6.
Subcellular localization of FBX proteins.
GFP-fused FBX proteins; At1g21410 (A), At1g21760 (B), At1g23390 (C), At1g30790 (D), At1g47730 (E), At1g64840 (F), At1g67190 (G), At2g24250 (H), At2g25490 (I and J), At3g03360 (K), At3g04660 (L), At3g24760 (M), At4g02440 (N), At4g05460 (O and P), At4g27050 (Q and R), At5g21040 (S and T) and At5g52880 (U) were observed. BF, bright field; IF, intrinsic fluorescence.